상세정보

This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Inclusive and authoritative, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations will be an invaluable resource to both basic and clinical practitioners in stem cell biology.

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This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Inclusive and authoritative, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations will be an invaluable resource to both basic and clinical practitioners in stem cell biology.

정보제공 :

CONTENTS
Preface = v
Contributors = ix
Therapeutic Mesenchymal Stromal Cells : Where We Are Headed / Patrick J. Hanley = 1
The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks / Vincent C. Chen ; Larry A. Couture = 13
Scalable Expansion of Human-Induced Pluripotent Stem Cells in Xeno-Free Microcarriers / Sara M. Badenes ; Tiago G. Fernandes ; Carlos A.V. Rodrigues ; Maria Margarida Diogo ; Joaquim M.S. Cabral = 23
Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs / Jens Durruthy Durruthy ; Vittorio Sebastiano = 31
Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging / Sébastien Sart ; Fabian Calixto Bejarano ; Yuanwei Yan ; Samuel C. Grant ; Yan Li = 43
Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells / Gema Martin-Manso ; Patrick J. Hanley = 53
Isolation and Expansion of Mesenchymal Stromal／Stem Cells from Umbilical Cord Under Chemically Defined Conditions / Heba Badraiq ; Liani Devito ; Dusko Ilic = 65
Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use / Phuc Van Pham ; Ngoc Kim Phan = 73
Xeno-Free Culture of Human Periodontal Ligament Stem Cells / Oriana Trubiani ; Francesca Diomede = 87
GMP-Compliant Human Adipose Tissue-Derived Mesenchymal Stem Cells for Cellular Therapy / Hamid-Reza Aghayan ; Parisa Goodarzi ; Babak Arjman = 93
cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal／Stem Cells / Elena Veronesi ; Jorge Sans Burns ; Alba Murgia ; Olivia Candini ; Valeria Rasini ; Ilenia Mastrolia ; Fabio Catani ; Paolo Paolucci ; Massimo Dominici = 109
GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation / Bagher Larijani ; Hamid-Reza Aghayan ; Parisa Goodarzi ; Babak Arjmand = 123
Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting / Gonçalo M.C. Rodrigues ; Tiago G. Fernandes ; Carlos A.V. Rodrigues ; Joaquim M.S. Cabral ; Maria Margarida Diogo = 137
Scalable Ex Vivo Expansion of Human Mesenchymal Stem／Stromal Cells in Microcarrier-Based Stirred Culture Systems / Joana G. Carmelo ; Ana Fernandes-Platzgummer ; Joaquim M.S. Cabral ; Cláudia Lobato da Silva = 147
Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process / Marta Serra ; Livia Roseti ; Alessandra Bassi = 161
Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells / Livia Roseti ; Marta Serra ; Alessandra Bassi = 171
Culture of Human Limbal Epithelial Stem Cells on Tenon's Fibroblast Feeder-Layers : A Translational Approach / Gaia Scafetta ; Camilla Siciliano ; Giacomo Frati ; Elena De Falco = 187
Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements / Christiane L. Elseberg ; Denise Salzig ; Peter Czermak = 199
Index = 219