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This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.

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This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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1. Suppressor Screens in Arabidopsis

Xin Li and Yuelin Zhang

2. Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants

Heithem Ben Abdallah and Petra Bauer

3. DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants

David Pose and Levi Yant

4. Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology

Iraida Amaya, Jeremy Pillet, and Kevin M. Folta

5. Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation

Masahito Shikata and Hiroshi Ezura

6. Culture of the Tomato Micro-Tom Cultivar in Greenhouse

Christophe Rothan, Daniel Just, Lucie Fernandez, Isabelle Atienza, Patricia Ballias, and Martine Lemaire-Chamley

7. Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry

Elio Fantini and Giovanni Giuliano

8. A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications

Arnaldo L. Schapire and L. Maria Lois

9. Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function

Mengmeng Zhu, Byeong Wook Jeon, Sisi Geng, Yunqing Yu, Kelly Balmant, Sixue Chen, and Sarah M. Assmann

10. Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells

Yinglang Wan, Yiqun Xue, Ruili Li, and Jinxing Lin

11. Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins

Yasuhiro Kadota, Alberto P. Macho, and Cyril Zipfel

12. Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions

Natsumi Maruta, Yuri Trusov, and Jose R. Botella

13. Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana

Meral Tunc-Ozdemir, Yan Fu, and Alan M. Jones

14. Analysis of Protein-Lipid Interactions Using Purified C2 Domains

Jessica Perez-Sancho, Arnaldo L. Schapire, Miguel A. Botella, and Abel Rosado

15. Assessing Kinase Activity in Plants with In-Gel Kinase Assays

Pengcheng Wang and Jian-Kang Zhu

16. Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins

Yusuke Kamiyoshihara, Denise M. Tieman, and Harry J. Klee

17. Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae -Plant Interactions

Alberto P. Macho, Jose S. Rufian, Javier Ruiz-Albert, and Carmen R. Beuzon

18. Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus

Yuri Trusov, Ralf G. Dietzgen, Natsumi Maruta, and Jose R. Botella

19. Simultaneous Determination of Plant Hormones by GC-TOF-MS

Jose G. Vallarino and Sonia Osorio

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