상세정보

Part I Methods Focused on Cytology

1: Using Fluorescence Microscopy to Study MitosisSai K. Balchand, Barbara J. Mann, and Patricia Wadsworth

2: Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation
Bin He and Daniela Cimini

Part II Methods Focused on Microtubules and the Mitotic Spindle

3: Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts
Aaron C. Groen and Timothy J. Mitchison

4: Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro
Marija Zanic

정보제공 :

Part I Methods Focused on Cytology

1: Using Fluorescence
Microscopy to Study Mitosis Sai K. Balchand, Barbara J. Mann, and Patricia
Wadsworth

 2: Using Photoactivatable
GFP to Study Microtubule Dynamics and Chromosome Segregation
Bin He and Daniela Cimini

Part II Methods Focused on Microtubules and the
Mitotic Spindle

3: Purification and Fluorescent Labeling
of Tubulin from Xenopus Laevis Egg Extracts
Aaron C. Groen and Timothy J. Mitchison

4: Measuring
the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro
 Marija Zanic

5: Imaging
and Quantifying the Dynamics of Gamma-Tubulin at Microtubule Minus Ends in Mitotic Spindles
Nicolas Lecland and Jens Luders

 6:
Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy
Matthew King and Sabine Petry

 7: Encapsulation
of Xenopus Egg and Embryo Extract Spindle
Assembly Reactions in Synthetic Cell-Like Compartments
with Tunable Size
Matthew C. Good

 Part III Methods Focused on Kinetochores and the
Kinetochore-Microtubule Interface

8: In Vitro Kinetochore Assembly
Matthew D. D. Miell and Aaron F. Straight

9: Biochemical
and Structural Analysis of Kinetochore Histone-Fold Complexes
Tatsuya Nishino and Tatsuo Fukagawa

 10:
Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells
Keith F. DeLuca, Jacob A. Herman, and Jennifer G.
DeLuca

 11: Studying
Kinetochores In Vivo Using FLIM-FRET
Tae Yeon Yoo and Daniel J. Needleman

Section IV Methods Focused on the Spindle Pole

12: Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole
Bodies
Kimberly K. Fong, Beth Graczyk, and Trisha N. Davis

 13: A Cell-Free
System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome
Conversion
Rajesh Kumar Soni and Meng-Fu Bryan Tsou

 14: Assays
to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation
Vladimir Joukov, Johannes C. Walter, and Arcangela De
Nicolo

 Part V Methods Focused on the Cellular Functions of
Microtubule Motor Proteins

15: Analyzing
Spindle Positioning Dynamics in Cultured Cells
Tomomi Kiyomitsu

 16:
Quantification of Mitotic Chromosome Alignment
Cindy Fonseca and Jason Stumpff

 17: Imaging Mitosis
in the Moss Physcomitrella Patens
 Moe Yamada, Tomohiro Miki, and Gohta Goshima

 18: Small Molecule
Approach to Study the Function of Mitotic Kinesins
Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer

 Part VI Novel Approaches to Study Spindle Function and
Regulation

19: Identification
and Characterization of Mitotic Spindle-Localized Transcripts
Amy B. Emerman, Ashwini Jambhekar, and Michael D.
Blower

 20: Probing Mitosis
by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible
Dimerization
Edward R. Ballister and Michael A. Lampson

 21: Studying
Kinetochore Kinases
Adrian T. Saurin and Geert J.P.L. Kops

 22: Engineering
and Functional Analysis of Mitotic Kinases through Chemical Genetics
Mathew J.K. Jones and Prasad V. Jallepalli

 Part VII The Mitotic Spindle and Cancer

23: Using Cell
Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer
Mijung Kwon

 24: Generation
and Purification of Tetraploid Cells
Elizabeth M. Shenk and Neil J. Ganem

 25: Anti-Microtubule
Drugs
Stefan Florian and Timothy J. Mitchison 

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