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Zygotic genome activation : methods and protocols

Zygotic genome activation : methods and protocols

자료유형
단행본
개인저자
Lee, Kiho.
서명 / 저자사항
Zygotic genome activation : methods and protocols / edited by Kiho Lee.
발행사항
New York, NY :   Humana Press :   Springer,   c2017.  
형태사항
xi, 272 p. : ill. (some col.) ; 27 cm.
총서사항
Springer protocols
ISBN
9781493969869 (print) 9781493969883 (electronic bk.) 1493969889 (electronic bk.)
내용주기
Clearance of maternal RNAs : not a Mummy's embryo anymore / Antonio Marco -- Link of zygotic genome activation and cell cycle control / Boyang Liu and Jörg Grosshans -- Role of microRNAs in zygotic genome activation : modulation of mRNA during embryogenesis / Alessandro Rosa and Ali H. Brivanlou -- Gene expression analysis in mammalian oocytes and embryos by quantitative real-time RT-PCR / Kyeoung-Hwa Kim, Su-Yeon Lee, and Kyung-Ah Lee -- Detection of miRNA in mammalian oocytes and embryos / Malavika K. Adur, Benjamin J. Hale, and Jason W. Ross -- Detection of bidirectional promoter-derived lncRNAs from small-scale samples using pre-amplification-free directional RNA-seq Method / Nobuhiko Hamazaki, Kinichi Nakashima, Katsuhiko Hayashi, and Takuya Imamura -- Detection and characterization of small noncoding RNAs in mouse gametes and embryos prior to zygotic genome activation / Jesús García-López, Eduardo Larriba, and Jesús del Mazo -- Purification of zygotically transcribed RNA through metabolic labeling of early zebrafish embryos / Patricia Heyn and Karla M. Neugebauer -- RNA FISH to study zygotic genome activation in early mouse embryos / Noémie Ranisavljevic, Ikuhiro Okamoto, Edith Heard, and Katia Ancelin -- Detection of RNA polymerase II in mouse embryos during zygotic genome activation using immunocytochemistry / Irina O. Bogolyubova and Dmitry S. Bogolyubov -- Immunological staining of global changes in DNA methylation in the early mammalian embryo / Yan Li and Christopher O'Neill -- Single cell restriction enzyme-based analysis of methylation at genomic imprinted regions in preimplantation mouse embryos / Ka Yi Ling, Lih Feng Cheow, Stephen R. Quake, William F. Burkholder, and Daniel M. Messerschmidt -- Use of chemicals to inhibit DNA replication, transcription, and protein synthesis to study zygotic genome activation / Kyungjun Uh and Kiho Lee -- Targeted gene knockdown in early embryos using siRNA / Lu Zhang and Zoltan Machaty -- Generating mouse models using zygote electroporation of nucleases (ZEN) technology with high efficiency and throughput / Wenbo Wang, Yingfan Zhang, and Haoyi Wang -- CRISPR/Cas9-mediated gene targeting during embryogenesis in swine / Junghyun Ryu and Kiho Lee -- Potential involvement of SCF-complex in zygotic genome activation during early bovine embryo development / Veronika Benesova, Veronika Kinterova, Jiri Kanka, and Tereza Toralova -- Use of histone K-M mutants for the analysis of transcriptional regulation in mouse zygotes / Keisuke Aoshima, Takashi Kimura, and Yuki Okada.
서지주기
Includes bibliographical references and index.
일반주제명
Developmental genetics --Laboratory manuals. Zygotes --Laboratory manuals. Gene expression --Laboratory manuals. Zygote. Transcriptional Activation. Gene Expression.
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020 ▼a 1493969889 (electronic bk.)
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245 0 0 ▼a Zygotic genome activation : ▼b methods and protocols / ▼c edited by Kiho Lee.
260 ▼a New York, NY : ▼b Humana Press : ▼b Springer, ▼c c2017.
300 ▼a xi, 272 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Springer protocols
490 1 ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 1605
504 ▼a Includes bibliographical references and index.
505 0 0 ▼t Clearance of maternal RNAs : not a Mummy's embryo anymore / ▼r Antonio Marco -- ▼t Link of zygotic genome activation and cell cycle control / ▼r Boyang Liu and Jörg Grosshans -- ▼t Role of microRNAs in zygotic genome activation : modulation of mRNA during embryogenesis / ▼r Alessandro Rosa and Ali H. Brivanlou -- ▼t Gene expression analysis in mammalian oocytes and embryos by quantitative real-time RT-PCR / ▼r Kyeoung-Hwa Kim, Su-Yeon Lee, and Kyung-Ah Lee -- ▼t Detection of miRNA in mammalian oocytes and embryos / ▼r Malavika K. Adur, Benjamin J. Hale, and Jason W. Ross -- ▼t Detection of bidirectional promoter-derived lncRNAs from small-scale samples using pre-amplification-free directional RNA-seq Method / ▼r Nobuhiko Hamazaki, Kinichi Nakashima, Katsuhiko Hayashi, and Takuya Imamura -- ▼t Detection and characterization of small noncoding RNAs in mouse gametes and embryos prior to zygotic genome activation / ▼r Jesús García-López, Eduardo Larriba, and Jesús del Mazo -- ▼t Purification of zygotically transcribed RNA through metabolic labeling of early zebrafish embryos / ▼r Patricia Heyn and Karla M. Neugebauer -- ▼t RNA FISH to study zygotic genome activation in early mouse embryos / ▼r Noémie Ranisavljevic, Ikuhiro Okamoto, Edith Heard, and Katia Ancelin -- ▼t Detection of RNA polymerase II in mouse embryos during zygotic genome activation using immunocytochemistry / ▼r Irina O. Bogolyubova and Dmitry S. Bogolyubov -- ▼t Immunological staining of global changes in DNA methylation in the early mammalian embryo / ▼r Yan Li and Christopher O'Neill -- ▼t Single cell restriction enzyme-based analysis of methylation at genomic imprinted regions in preimplantation mouse embryos / ▼r Ka Yi Ling, Lih Feng Cheow, Stephen R. Quake, William F. Burkholder, and Daniel M. Messerschmidt -- ▼t Use of chemicals to inhibit DNA replication, transcription, and protein synthesis to study zygotic genome activation / ▼r Kyungjun Uh and Kiho Lee -- ▼t Targeted gene knockdown in early embryos using siRNA / ▼r Lu Zhang and Zoltan Machaty -- ▼t Generating mouse models using zygote electroporation of nucleases (ZEN) technology with high efficiency and throughput / ▼r Wenbo Wang, Yingfan Zhang, and Haoyi Wang -- ▼t CRISPR/Cas9-mediated gene targeting during embryogenesis in swine / ▼r Junghyun Ryu and Kiho Lee -- ▼t Potential involvement of SCF-complex in zygotic genome activation during early bovine embryo development / ▼r Veronika Benesova, Veronika Kinterova, Jiri Kanka, and Tereza Toralova -- ▼t Use of histone K-M mutants for the analysis of transcriptional regulation in mouse zygotes / ▼r Keisuke Aoshima, Takashi Kimura, and Yuki Okada.
650 0 ▼a Developmental genetics ▼v Laboratory manuals.
650 0 ▼a Zygotes ▼v Laboratory manuals.
650 0 ▼a Gene expression ▼v Laboratory manuals.
650 2 ▼a Zygote.
650 2 ▼a Transcriptional Activation.
650 2 ▼a Gene Expression.
655 2 ▼a Laboratory Manuals.
700 1 ▼a Lee, Kiho.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v 1605.
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 571.85 Z99 등록번호 121240841 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

책소개

Preface...
Table of Contents...
Contributing Authors...


1. Clearance of Maternal RNAs: Not a Mummy's Embryo Anymore
Antonio Marco

2. Link of Zygotic Genome Activation and Cell Cycle Control
Boyang Liu and J?rg Grosshans

3. Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis
Alessandro Rosa and Ali H. Brivanlou

4. Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR
Kyeoung-Hwa Kim, Su-Yeon Lee, and Kyung-Ah Lee

5. Detection of miRNA in Mammalian Oocytes and Embryos
Malavika K. Adur, Benjamin J. Hale, and Jason W. Ross

6. Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method
Nobuhiko Hamazaki, Kinichi Nakashima, Katsuhiko Hayashi, and Takuya Imamura

7. Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation
Jes?s Garc?a-L?pez, Eduardo Larriba, and Jes?s del Mazo

8. Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
Patricia Heyn and Karla M. Meugebauer

9. RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos
No?mie Ranisavljevic, Ikuhiro Okamoto, Edith Heard, and Katia Ancelin

10. Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry
Irina O. Bogolyubova and Dmitry S. Bogolyubov

11. Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo
Yan Li and Christopher O'Neill

12. Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos
Ka Yi Ling, Lih Fenh Cheow, Stephen R. Quake, William F. Burkholder, and Daniel M. Messerschmidt

13. Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation
Kyungjun Uh and Kiho Lee

14. Targeted Gene Knockdown in Early Embryos using siRNA
Lu Zhang and Zoltan Machaty

15. Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput
Wenbo Wang, Yingfan Zhang, and Haoyi Wang

16. CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine
Junghyun Ryu and Kiho Lee

17. Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development
Veronika Benesova, Veronika Kinterova, Jiri Kanka, and Tereza Toralova

18. Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes
Keisuke Aoshima, Takashi Kimura, and Yuki Okada


정보제공 : Aladin

목차

Clearance of Maternal RNAs: Not a Mummy’s Embryo Anymore
Link of Zygotic Genome Activation and Cell Cycle Control
Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis
Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR
Detection of miRNA in Mammalian Oocytes and Embryos
Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method
Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation
Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos
Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry
Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo
Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos
Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation
Targeted Gene Knockdown in Early Embryos using siRNA
Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput
CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine
Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development
Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes.

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