상세정보

1. 3D Cell Culture: An Introduction

Zuzana Koledova

Part I: Hydrogels and Scaffolds for 3D Cell Culture

Bryan N. Brown, Michael J. Buckenmeyer, and Travis A. Prest

3. 3D Cell Culture in Interpenetrating Networks of Alginate and rBM Matrix

Katrina Wisdom and Ovijit Chaudhuri

4. Hydrogel-Based In Vitro Models of Tumor Angiogenesis

Laura J. Bray, Marcus Binner, Uwe Freudenberg, and Carsten Werner

5. Generation of Induced Pluripotent Stem Cells in Defined Three-Dimensional PEG Hydrogels

Massimiliano Caiazzo, Yoji Tabata, and Matthias Lutolf

Edgar B. Montufar, Lucy Vojtova, Ladislav Celko, and Maria-Pau Ginebra

Part II: 3D Organoid and Organotypic Cultures

7. Establishment of 3D Intestinal Organoid Cultures from Intestinal Stem Cells

Shinya Sugimoto and Toshiro Sato

8. 3D Co-Culture of Mammary Organoids with Fibrospheres: A Model for Studying Epithelial-Stromal Interactions During Mammary Branching Morphogenesis

Zuzana Koledova

9. An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis

Jelena R. Linnemann, Lisa K. Meixner, Haruko Miura, and Christina H. Scheel

10. 3D Primary Culture Model to Study Human Mammary Development

Daniel H. Miller, Ethan S. Sokol, and Piyush B. Gupta

11. Lungosphere Assay: 3D Culture of Lung Epithelial Stem/Progenitor Cells

Anas Rabata, Ales Hampl, and Zuzana Koledova

12. 3D Hanging Drop Culture to Establish Prostate Cancer Organoids

Theresa Eder and Iris E. Eder

13. 3D Fluid-Dynamic Culture Models of Multiple Myeloma

Marina Ferrarini, Nathalie Steimberg, Jennifer Boniotti, Angiola Berenzi, Daniela Belloni, Giovanna Mazzoleni, and Elisabetta Ferrero

14. Preparation of a Three-Dimensional Full Thickness Skin Equivalent

Christian Reuter, Heike Walles, and Florian Groeber

15. Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System

Romana E. Ranftl and Fernando Calvo

16. 3D Co-Culture Model of the Brain Parenchyma/Metastasis Interface of Brain Metastasis

Raquel Blazquez and Tobias Pukrop

Part III: Micropatterning

정보제공 :

Preface	p. v
Contributors	p. ix
1	3D Cell Culture: A Review of Current Approaches and Techniques   John W. Haycock	p. 1
2	Scaffolds for Tissue Engineering and 3D Cell Culture   Eleonora Carletti and Antonella Motta and Claudio Migliaresi	p. 17
3	Tracking Nanoparticles in Three-Dimensional Tissue-Engineered Models Using Confocal Laser Scanning Microscopy   Vanessa Hearnden and Sheila MacNeil and Giuseppe Battaglia	p. 41
4	Using Immuno-Scanning Electron Microscopy for the Observation of Focal Adhesion-substratum interactions at the Nano- and Microscale in S-Phase Cells   ManusJ.P. Biggs and R. Geoff Richards and Matthew J. Dalby	p. 53
5	3D Sample Preparation for Orthopaedic Tissue Engineering Bioreactors   Sarah H. Cartmell and Sarah Rathbone and Gemma Jones and L. Araida Hidalgo-Bastida	p. 61
6	Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs   David A. Lee and June Brand and Donald Salter and Oto-Ola Akanji and Tina T. Chowdhury	p. 77
7	Genetic Modification of Chondrocytes Using Viral Vectors   Teresa Coughlan and Aileen Crawford and Paul Hatton and Michael Barker	p. 99
8	Stem Cell and Neuron Co-cultures for the Study of Nerve Regeneration   Paul J. Kingham and Cristina Mantovani and Giorgio Terenghi	p. 115
9	Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use   Sheila MacNeil and Joanna Shepherd and Louise Smith	p. 129
10	Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for Peripheral Nerve Repair   Celia Murray-Dunning and Sally L. McArthur and Tao Sun and Rob McKean and Anthony J. Ryan and John W. Haycock	p. 155
11	Encapsulation of Human Articular Chondrocytes into 3D Hydrogel: Phenotype and Genotype Characterization   Rui C. Pereira and Chiara Gentili and Ranieri Cancedda and Helena S. Azevedo and Rui L. Reis	p. 167
12	Micro-structured Materials and Mechanical Cues in 3D Collagen Gels   James B. Phillips and Robert Brown	p. 183
13	Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract   Claire L. Varley and Jennifer Southgate	p. 197
14	Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial-Neuroectodermal-Mesenchymal Interaction System   Desmond J. Tobin	p. 213
15	Human Endothelial and Osteoblast Co-cultures on 3D Biomaterials   Ronald E. Unger and Sven Halstenberg and Anne Sartoris and C. James Kirkpatrick	p. 229
16	Assessment of Nanomaterials Cytotoxicity and Internalization   Noha M. Zaki and Nicola Tirelli	p. 243
17	Practical Aspects of OCT Imaging in Tissue Engineering   Stephen J. Matcher	p. 261
18	Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture   Lee Buttery and Robert Bielby and Daniel Howard and Kevin Shakesheff	p. 281
19	3D Structuring of Biocompatible and Biodegradable Polymers Via Stereolithography   Andrew A. Gill and Frederik Claeyssens	p. 309
20	Alvetex": Polystyrene Scaffold Technology for Routine Three Dimensional Cell Culture   Eleanor Knight and Bridgid Murray and Ross Carnachan and Stefan Przyborski	p. 323
Index	p. 341