상세정보

PART I: Protocols for preparing primary cultures, cell lines and engineered Schwann cells

1. Isolation of Schwann Cell Precursors from Rodents
Rhona Mirsky and Kristjan R. Jessen

2. Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies

Patrice Maurel

3. Isolation and Expansion of Schwann Cells from Transgenic Mouse Models
Jihyun Kim and Haesun Kim

4. Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers

Natalia D. Andersen and Paula V. Monje

5. Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells

6. Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations

Kristine M. Ravelo, Natalia D. Andersen, and Paula V. Monje

7. Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies

Adriana E. Brooks, Gagani Athauda, Mary Bartlett Bunge, and Aisha Khan

8. Chemical Conversion of Human Fibroblasts into functional Schwann Cells

Eva C. Thoma

9. Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats

Y.P. Tsui, Graham K. Shea, Y.S. Chan, and Daisy K. Shum

10. Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells

Sa Cai, Daisy KY Shum, and Ying-Shing Chan

11. Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay
Alejandra M. Petrilli and Cristina Fern?ndez-Valle

PART II: Protocols for studying Schwann cells behavior and myelination in vitro

12. Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies

Corey Heffernan and Patrice Maurel

Vania W. Almeida, Margaret L. Bates, and Mary Bartlett Bunge

14. Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures

Paula V. Monje

15. The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes

Yannick Poitelon and M. Laura Feltri

16. In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia

Rodrigo L?pez-Leal, Paula Diaz, and Felipe A. Court

Susana R. Cerqueira, Yee-Shuan Lee, and Mary Bartlett Bunge

18. Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development
Mateusz M. Urbanski and Carmen V. Melendez-Vasquez

19. Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs

Cristian De Gregorio, Paula D?az, Rodrigo L?pez-Leal, Patricio Manque, and Felipe A. Court

20. 3D Cancer Migration Assay with Schwann Cells

Laura Fangmann, Steffen Teller, Pavel Stupakov, Helmut Friess, G?ralp O. Ceyhan, and Ihsan Ekin Demir

PART III: Protocols for studying Schwann cell development and myelination in viv

정보제공 :

Isolation of Schwann Cell Precursors from Rodents
Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies
Isolation and Expansion of Schwann Cells from Transgenic Mouse Models
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers
Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells
Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations
Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies
Chemical Conversion of Human Fibroblasts into functional Schwann Cells
Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats
Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells
Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay
Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies
Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis
Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures
The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes
In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia
A Culture Model To Study Neuron – Schwann cell – Astrocyte Interactions
Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development
Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs
3D Cancer Migration Assay with Schwann Cells
Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis
Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration
The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin
Analysis of Myelinating Schwann Cells in Human Skin Biopsies
Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae
Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin
Live Imaging of Schwann Cell Development in Zebra Fish
Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord
Schwann Cell Transplantation Methods Using Biomaterials
Viral Transduction of Schwann Cells for Peripheral Nerve Repair
Intraspinal Delivery of Schwann Cells for Spinal Cord Injury. .