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Molecular motors : methods and protocols / 2nd ed

Molecular motors : methods and protocols / 2nd ed

자료유형
단행본
개인저자
Lavelle, Christophe.
서명 / 저자사항
Molecular motors : methods and protocols / edited by Christophe Lavelle.
판사항
2nd ed.
발행사항
New York, NY :   Humana Press :   Springer,   2018.  
형태사항
xii, , 452 p. : ill. (some col.) ; 27 cm.
총서사항
Methods in Molecular Biology,1064-3745 ; 1805
ISBN
9781493985562 1493985566 149398554X 9781493985548
요약
This volume expands on the previous edition with a more extensive look at molecular motors and their roles in muscle contractions, vesicle transport, flagellar beating, chromosome segregation, and DNA replication and repair. The chapters in this book are divided into three parts: Part One looks at membrane motors, such as the bacterial flagellar rotary motor; Part Two discusses cytoskeletal motors, such as kinesin and myosin; and Part Three talks about nucleic acid motors, such as DNA polymerases, helicases, and nucleosome remodelers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Molecular Motors: Methods and Protocols, Second Edition is a valuable resource for (bio)physicists and molecular/cellular biologists whose research delves into the mechanisms at work in cells and the motors which power them.
내용주기
Cellular and Nuclear Forces: An Overview -- The Bacterial Flagellar Rotary Motor in Action -- Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase -- Using Microfluidics Single Filament Assay to Study Formin Control of Actin Assembly -- Engineering Synthetic Myosin Filaments using DNA Nanotubes -- Direct Imaging of Walking Myosin V by High-Speed Atomic Force Microscopy -- High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates -- Multicolor Tracking of Molecular Motors at Nanometer Resolution -- High-Speed Optical Tweezers for the Study of Single Molecular Motors -- Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force Microscopy -- The Role of Supercoiling in the Motor Activity of RNA Polymerases -- Single-Molecule FRET Analysis of Replicative Helicases -- Recombinases and Related Proteins in the Context of Homologous Recombination Analyzed by Molecular Microscopy -- DNA Organization and Segregation Super-Resolved -- Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase -- Single Molecule Angular Optical Trapping for Studying Transcription under Torsion -- Anisotropy-Based Nucleosome Repositioning Assay -- Remodeling and Repositioning of Nucleosomes in Nucleosomal Arrays -- Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers -- Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, lambda, and T4 with Optical Tweezers -- Methods for Single Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging Motor.
서지주기
Includes bibliographical references and index.
일반주제명
Life sciences. Cytology.
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020 ▼a 9781493985562
020 ▼a 1493985566
020 ▼a 149398554X
020 ▼a 9781493985548
035 ▼a (OCoLC)1048007463 ▼z (OCoLC)1043866408
040 ▼a AU@ ▼b eng ▼c AU@ ▼d YDX ▼d OCLCO ▼d OCLCF ▼d KUB ▼d 211009
049 ▼a KUBA
050 4 ▼a QH573-671
082 0 4 ▼a 571.6 ▼2 23
084 ▼a 571.6 ▼2 DDCK
090 ▼a 571.6 ▼b M718-2
245 0 0 ▼a Molecular motors : ▼b methods and protocols / ▼c edited by Christophe Lavelle.
250 ▼a 2nd ed.
260 ▼a New York, NY : ▼b Humana Press : ▼b Springer, ▼c 2018.
300 ▼a xii, , 452 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Methods in Molecular Biology, ▼x 1064-3745 ; ▼v 1805
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
505 0 ▼a Cellular and Nuclear Forces: An Overview -- The Bacterial Flagellar Rotary Motor in Action -- Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase -- Using Microfluidics Single Filament Assay to Study Formin Control of Actin Assembly -- Engineering Synthetic Myosin Filaments using DNA Nanotubes -- Direct Imaging of Walking Myosin V by High-Speed Atomic Force Microscopy -- High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates -- Multicolor Tracking of Molecular Motors at Nanometer Resolution -- High-Speed Optical Tweezers for the Study of Single Molecular Motors -- Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force Microscopy -- The Role of Supercoiling in the Motor Activity of RNA Polymerases -- Single-Molecule FRET Analysis of Replicative Helicases -- Recombinases and Related Proteins in the Context of Homologous Recombination Analyzed by Molecular Microscopy -- DNA Organization and Segregation Super-Resolved -- Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase -- Single Molecule Angular Optical Trapping for Studying Transcription under Torsion -- Anisotropy-Based Nucleosome Repositioning Assay -- Remodeling and Repositioning of Nucleosomes in Nucleosomal Arrays -- Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers -- Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, lambda, and T4 with Optical Tweezers -- Methods for Single Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging Motor.
520 ▼a This volume expands on the previous edition with a more extensive look at molecular motors and their roles in muscle contractions, vesicle transport, flagellar beating, chromosome segregation, and DNA replication and repair. The chapters in this book are divided into three parts: Part One looks at membrane motors, such as the bacterial flagellar rotary motor; Part Two discusses cytoskeletal motors, such as kinesin and myosin; and Part Three talks about nucleic acid motors, such as DNA polymerases, helicases, and nucleosome remodelers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Molecular Motors: Methods and Protocols, Second Edition is a valuable resource for (bio)physicists and molecular/cellular biologists whose research delves into the mechanisms at work in cells and the motors which power them.
650 0 ▼a Life sciences.
650 0 ▼a Cytology.
700 1 ▼a Lavelle, Christophe.
830 0 ▼a Methods in molecular biology ; ▼v 1805.
830 0 ▼a Springer protocols.
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 571.6 M718-2 등록번호 121246656 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

목차

Cellular and Nuclear Forces: An Overview
The Bacterial Flagellar Rotary Motor in Action
Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase
Using Microfluidics Single Filament Assay to Study Formin Control of Actin Assembly
Engineering Synthetic Myosin Filaments using DNA Nanotubes
Direct Imaging of Walking Myosin V by High-Speed Atomic Force Microscopy
High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates
Multicolor Tracking of Molecular Motors at Nanometer Resolution
High-Speed Optical Tweezers for the Study of Single Molecular Motors
Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force Microscopy
The Role of Supercoiling in the Motor Activity of RNA Polymerases
Single-Molecule FRET Analysis of Replicative Helicases
Recombinases and Related Proteins in the Context of Homologous Recombination Analyzed by Molecular Microscopy
DNA Organization and Segregation Super-Resolved
Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase
Single Molecule Angular Optical Trapping for Studying Transcription under Torsion
Anisotropy-Based Nucleosome Repositioning Assay
Remodeling and Repositioning of Nucleosomes in Nucleosomal Arrays
Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers
Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, lambda, and T4 with Optical Tweezers
Methods for Single Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging Motor.

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