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Plant innate immunity : methods and protocols

Plant innate immunity : methods and protocols (1회 대출)

자료유형
단행본
개인저자
Gassmann, Walter.
서명 / 저자사항
Plant innate immunity : methods and protocols / edited by Walter Gassmann.
발행사항
New York, NY :   Humana Press :   Springer,   2019.  
형태사항
xiii, 260 p. : ill. (some col.) ; 27 cm.
총서사항
Springer protocols
ISBN
9781493994571 1493994573
요약
Back Cover Copy The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.
내용주기
Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains -- Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in planta -- Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA -- A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions -- Quantification of Extracellular ATP in Plant Suspension Cell Cultures -- Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions -- Method to Study Dynamics of Membrane-Bound Plant Transcription Factors Biotic Interactions in Tomato -- Preparation of Plant Material for Analysis of Proteinduring-Nucleic Acid Interactions by FRET-FLIM -- Analysis of DNA Methylation Profile in Plants by Chop-PCR -- Change in Nucleosome Dynamics during Stress Responses in Plants -- Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry -- A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments -- Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae -- Quantification of Cauline Leaf Abscission in Response to Plant Pathogens -- Methods for Replicating Leaf Vibrations Induced by Insect Herbivores -- Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes -- The Apple Fruitlet Model System for Fire Blight Disease -- Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins -- Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems -- Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants -- Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth -- A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance.
서지주기
Includes bibliographical references and index.
일반주제명
Plant immunology. Plants --Disease and pest resistance. Botany.
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082 0 4 ▼a 571.9/62 ▼2 23
084 ▼a 571.962 ▼2 DDCK
090 ▼a 571.962 ▼b P714
245 0 0 ▼a Plant innate immunity : ▼b methods and protocols / ▼c edited by Walter Gassmann.
260 ▼a New York, NY : ▼b Humana Press : ▼b Springer, ▼c 2019.
300 ▼a xiii, 260 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Springer protocols
490 1 ▼a Methods in molecular biology, ▼x 1940-6029 ; ▼v 1991
504 ▼a Includes bibliographical references and index.
505 0 ▼a Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains -- Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in planta -- Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA -- A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions -- Quantification of Extracellular ATP in Plant Suspension Cell Cultures -- Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions -- Method to Study Dynamics of Membrane-Bound Plant Transcription Factors Biotic Interactions in Tomato -- Preparation of Plant Material for Analysis of Proteinduring-Nucleic Acid Interactions by FRET-FLIM -- Analysis of DNA Methylation Profile in Plants by Chop-PCR -- Change in Nucleosome Dynamics during Stress Responses in Plants -- Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry -- A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments -- Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae -- Quantification of Cauline Leaf Abscission in Response to Plant Pathogens -- Methods for Replicating Leaf Vibrations Induced by Insect Herbivores -- Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes -- The Apple Fruitlet Model System for Fire Blight Disease -- Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins -- Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems -- Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants -- Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth -- A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance.
520 ▼a Back Cover Copy The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.
650 0 ▼a Plant immunology.
650 0 ▼a Plants ▼x Disease and pest resistance.
650 0 ▼a Botany.
700 1 ▼a Gassmann, Walter.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v.1991.
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 571.962 P714 등록번호 121249830 (1회 대출) 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

책소개

The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.




New feature

Back Cover Copy

The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.




정보제공 : Aladin

목차

Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains
Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in planta
Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA
A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions
Quantification of Extracellular ATP in Plant Suspension Cell Cultures
Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions
Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in Tomato
Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET-FLIM
Analysis of DNA Methylation Profile in Plants by Chop-PCR
Change in Nucleosome Dynamics during Stress Responses in Plants
Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry
A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments
Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae
Quantification of Cauline Leaf Abscission in Response to Plant Pathogens
Methods for Replicating Leaf Vibrations Induced by Insect Herbivores
Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes
The Apple Fruitlet Model System for Fire Blight Disease
Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins
Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems
Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants
Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth
A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance.

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