| 000 | 01336camuu2200337 a 4500 | |
| 001 | 000045535452 | |
| 005 | 20111219170209 | |
| 008 | 070907s2008 njua b 001 0 eng | |
| 010 | ▼a 2007037114 | |
| 020 | ▼a 9780470089934 (cloth) | |
| 035 | ▼a (KERIS)REF000013567526 | |
| 040 | ▼a DNLM/DLC ▼c DLC ▼d DLC ▼d 211009 | |
| 042 | ▼a pcc | |
| 050 | 0 0 | ▼a RB36 ▼b .C87 2008 |
| 060 | 1 0 | ▼a QY 25 ▼b C976 2008 |
| 082 | 0 0 | ▼a 610.28/4 ▼2 22 |
| 084 | ▼a 610.284 ▼2 DDCK | |
| 090 | ▼a 610.284 ▼b C976 | |
| 245 | 0 0 | ▼a Current protocols essential laboratory techniques / ▼c edited by Sean R. Gallagher, Emily A. Wiley. |
| 260 | ▼a Hoboken : ▼b John Wiley & Sons, ▼c c2008. | |
| 300 | ▼a 1 v. (various pagings) : ▼b ill. ; ▼c 28 cm. | |
| 504 | ▼a Includes bibliographical references and index. | |
| 505 | 0 | ▼a Volume/weight measurement -- Concentration measurement -- Reagent preparation -- Cell culture techniques -- Sample preparation -- Chromatography -- Electrophoresis -- Blotting -- Microscopy -- Enzymatic reactions. |
| 650 | 0 | ▼a Medical laboratories. |
| 650 | 0 | ▼a Diagnosis, Laboratory |
| 650 | 1 2 | ▼a Laboratory Techniques and Procedures. |
| 650 | 2 2 | ▼a Clinical Laboratory Techniques. |
| 650 | 2 2 | ▼a Clinical Protocols. |
| 700 | 1 | ▼a Gallagher, Sean R. |
| 700 | 1 | ▼a Wiley, Emily A. |
소장정보
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 과학도서관/Sci-Info(2층서고)/ | 청구기호 610.284 C976 | 등록번호 121215895 (2회 대출) | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
| No. 2 | 소장처 의학도서관/자료실(3층)/ | 청구기호 610.284 C976 | 등록번호 131034738 (2회 대출) | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 과학도서관/Sci-Info(2층서고)/ | 청구기호 610.284 C976 | 등록번호 121215895 (2회 대출) | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 의학도서관/자료실(3층)/ | 청구기호 610.284 C976 | 등록번호 131034738 (2회 대출) | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
컨텐츠정보
책소개
2008 PROSE award winner for Excellence in Biology and the Life Sciences
From the leading branded source for methods in the life sciences, this essential resource for the lab provides every researcher with the skills and understanding of fundamental laboratory procedures to ensure greater success at the bench. It takes the novice researcher from the very basics of reagent preparation to the use of routine instrumentation found in most life science research laboratories around the world.
- Developed by Current Protocols, the most trusted and recognized source of protocols
- Teaches new investigators how to perform basic laboratory research techniques
- Describes solution chemistry and preparation
- Covers basic laboratory safety
- Describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes
- Teaches how to manage information from lab notebooks, images, literature references, as well as manuscript preparation
This book is an invaluable resource for researchers in all areas of the life sciences, especially molecular biology, biotechnology, genetics, and immunology. It is essential reading for undergraduate and graduate students alike and is also of interest for investigators new to life science research.
정보제공 :
목차
Foreword.
Preface.
Contributors.
Common Conversion Factors.
Combining Techniques to Answer Molecular Questions.
Chapter 1: Volume/Weight Measurement.
1.1 Volume Measurement.
1.2 Weight Measurement.
Chapter 2: Concentration Measurement.
2.1 Spectrophotometry.
2.2 Quantitation of Nucleic Acids and Proteins.
2.3 Radiation Safety and Measurement.
Chapter 3: Reagent Preparation.
3.1 Reagent Preparation: Theoretical and Practical Discussions.
3.2 Measurement of pH.
3.3 Recipes for Commonly Encountered Reagents.
Chapter 4: Cell Culture Techniques.
4.1 Aseptic Technique.
4.2 Culture of Escherichia coli and Related Bacteria.
Chapter 5: Sample Preparation.
5.1 Centrifugation.
5.2 Purification and Concentration of Nucleic Acids.
Chapter 6: Chromatography.
6.1 Overview of Chromatography.
6.2 Column Chromatography.
Chapter 7: Electrophoresis.
7.1 Overview of Electrophoresis.
7.2 Agarose Gel Electrophoresis.
7.3 SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
7.4 Staining Proteins in Gels.
7.5 Overview of Digital Electrophoresis Analysis.
Chapter 8: Blotting.
8.1 Overview of Blotting.
8.2 Nucleic Acid Blotting: Southern and Northern.
8.3 Protein Blotting: Immunoblotting.
8.4 Labeling DNA and Preparing Probes.
Chapter 9: Microscopy.
9.1 Conventional Light Microscopy.
9.2 Immunofluorescence Microscopy.
Chapter 10: Enzymatic Reactions.
10.1 Working with Enzymes.
10.2 Overview of PCR.
10.3 Real-Time PCR.
10.4 DNA Sequencing: An Outsourcing Guide.
Appendices.
A.1 Laboratory Safety.
A.2 Laboratory Notebooks and Data Storage.
A.3A Ethical Considerations When Altering Digital Images.
A.3B Practical Considerations When Altering Digital Images.
A.4 Statistical Analysis.
A.5A Preparing and Presenting a Poster.
A.5B Preparing and Presenting a Talk.
Index.
정보제공 :