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Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study.? The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances.? Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D.? It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.

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Cell migration is a key component of many biological processes. This book provides a catalogue of techniques for the study of cell migration. It brings together a wide range of these techniques from the basic migration assays to recent technical advances.?

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Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study.? The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances.? Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D.? It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.

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1. Cell Migration: An Overview

Miguel Vicente-Manzanares and Alan Rick Horwitz

2. Scratch-Wound Assay

Giles Cory

3. HGF-Induced DU145 Cell Scatter Assay

Sally T. Fram, Claire M. Wells, and Gareth E. Jones

4. Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time

Sanjay Chaubey, Anne J. Ridley, and Claire M. Wells

5. Imaging Cells Within 3D Cell Derived Matrix

Samantha J. King and Maddy Parsons

6. Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro

Daniel M. Suter

7. Boyden Chamber

Marco Falasca, Claudio Raimondi, and Tania Maffucci

8. Transwell® Invasion Assays

John Marshall

9. Imaging Podosome Dynamics and Matrix Degradation

Taylor W. Starnes, Christa L. Cortesio, and Anna Huttenlocher

10. Endothelial Cell Migration Under Flow

Beata Wojciak-Stothard

11. In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments

Michael Sixt

12. Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy

Robert J. Cain, Barbara Borda d'Agua, and Anne J. Ridley

13. Chemotaxis of Slow Migrating Mammalian Cells Analyzed by Video Microscopy

Roman Zantl and Elias Horn

14. Live Cell Fluorescence Microscopy Techniques

Shawn A. Galdeen and Alison J. North

15. Measuring Invasion in an Organotypic Model

Veronika Jenei, Maria L. Nystrom, and Gareth J. Thomas

16. Analysis of Cell Migration Using Caenorhabditis elegans as a Model System

Ming-Ching Wong, Maria Martynovsky, and Jean E. Schwarzbauer

17. Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration

Carolina G.A. Moreira, Jennifer C. Regan, Anna Zaidman-Remy, Antonio Jacinto, and Soren Prag

18. Measuring Inflammatory Cell Migration in the Zebrafish

Philip M. Elks, Catherine A. Loynes, and Stephen A. Renshaw

19. Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement

Mohit Prasad, Xiaobo Wang, Li He, and Denise J. Montell

20. Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants

Yulia Artemenko, Kristen F. Swaney, and Peter N. Devreotes

21. Simple Experimental and Spontaneous Metastasis Assays in Mice

Gary M. Box and Suzanne A. Eccles

22. Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour 

of Cancer Cells In Vivo

Sylvia E. Le Devedec, Wies van Roosmalen, Chantal Pont, Reshma Lalai, Hans de Bont, and Bob van de Water

23. Measuring Angiogenesis in Mice

Bernardo Tavora and Kairbaan Hodivala-Dilke

24. Time-Lapse Imaging of Chick Cardiac Precursor Cells

Junfang Song, Qiaoyun Yue, and Andrea Munsterberg

25. Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy

Juliane P. Schwarz, Ireen Konig, and Kurt I. Anderson

26. Fluorescence Recovery After Photobleaching

Alex Carisey, Matthew Stroud, Ricky Tsang, and Christoph Ballestrem

27. Measuring FRET Using Time-Resolved FLIM

Penny E. Morton and Maddy Parsons

28. Cell Migration in Confinement: A Micro-Channel Based Assay

Melina L. Heuze, Olivier Collin, Emmanuel Terriac, Ana-Maria Lennon-Dumenil, and Matthieu Piel

29. Functional Screening with a Live Cell Imaging Based Random Cell Migration Assay

Wies van Roosmalen, Sylvia E. Le Devedec, Sandra Zovko, Hans de Bont, and Bob van de Water

30. Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays

Eric Theveneau and Roberto Mayor

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