상세정보

1. Discovery of the Intracellular Signaling Molecule Cyclic di-GMP

Ute R?mling and Michael Y. Galperin

PARt I: Synthesis of c-di-GMP

2. Enzymatic Production of C-di-GMP Using a Thermophilic Diguanylate Cyclase

3. Synthesis of [³²P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations

Barbara I. Kazmierczak

Part II: Detection and Quantitation of c-di-GMP

4. High-performance Liquid Chromatography (HPLC)-based Detection and Quantitation of Cellular c-di-GMP

Heike B?hre and Volkhard Kaever

6. Detection of Cyclic Dinucleotides by STING

Xiao-Xia Du and Xiao-Dong Su

Geoffrey B. Severin and Christopher M. Waters

Part III: Visualizing c-di-GMP levels using biosensors

8. Gauging and Visualizing c-di-GMP Levels in Pseudomonas aeruginosa using Fluorescence-based Biosensors

Morten Rybtke, Song Lin Chua, Joey Kuok Hoong Yam, Michael Givskov, Liang Yang, and Tim Tolker-Nielsen

9. Cyclic di-GMP-responsive Transcriptional Reporter Bioassays in Pseudomonas aeruginosa

Bradley R. Borlee, Grace I. Borlee, Kevin H. Martin, and Yasuhiko Irie

10. Live Flow Cytometry Analysis of c-di-GMP levels in Single Cell Populations

Jongchan Yeo, Xin C. Wang, and Ming C. Hammond

Part IV: Indirect detection of c-di-GMP levels

11. Experimental Detection and Visualization of the Extracellular Matrix in Macrocolony Biofilms

Diego O. Serra and Regine Hengge

12. Congo Red Stain Identifies Matrix Overproduction and is an Indirect Measurement for c-di-GMP in many Species of Bacteria

Christopher J. Jones and Daniel J. Wozniak

13. Type IV Pili-dependent Motility as a Tool to Determine the Activity of c-di-GMP Modulating Enzymes in Myxococcus xanthus

Dorota Skotnicka and Lotte S gaard-Andersen

PART V: Modulation of c-di-GMP levels And bacterial responses

14. Using Light-Activated Enzymes for Modulating Intracellular c-di-GMP Levels in Bacteria

Min-Hyung Ryu, Anastasia Fomicheva, Lindsey O'Neal, Gladys Alexandre, and Mark Gomelsky

15. Analysis of c-di-GMP Levels Synthesized by a Photoreceptor Protein in Response to Different Light Qualities using an in vitro Enzymatic Assay

Veronika Angerer, Lars-Oliver Essen, and Annegret Wilde

16. Probing the Role of Cyclic di-GMP Signalling Systems in Disease using Chinese Radish

Shi-Qi An, Ji-Liang Tang, and J. Maxwell Dow

Ronan R McCarthy, Martina Valentini, and Alain Filloux

18. Semi-quantitative Analysis of the Red, Dry, and Rough Colony Morphology of Salmonella enterica serovar Typhimurium and Escherichia coli using Congo Red

Annika Cimdins and Roger Simm

Part VI: Measuring c-di-GMP modulating activities

19. Fluorescent 2-aminopurine c-di-GMP and GpG analogs as PDE Probes

Jie Zhou, Clement Opoku-Temeng, and Herman O. Sintim

정보제공 :

Discovery of the Intracellular Signaling Molecule Cyclic di-GMP
Enzymatic Production of C-di-GMP Using a Thermophilic Diguanylate Cyclase
Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations
High-performance Liquid Chromatography (HPLC)-based Detection and Quantitation of Cellular c-di-GMP
Identification and Quantification of Cyclic Di-Guanosine Monophosphate and its Linear Metabolites by Reversed-Phase LC-MS/MS
Detection of Cyclic Dinucleotides by STING
Spectrophotometric and Mass Spectroscopic Methods for the Quantification and Kinetic Evaluation of In Vitro c-di-GMP Synthesis
Gauging and Visualizing c-di-GMP Levels in Pseudomonas aeruginosa using Fluorescence-based Biosensors
Cyclic di-GMP-responsive Transcriptional Reporter Bioassays in Pseudomonas aeruginosa
Live flow cytometry analysis of c-di-GMP levels in Single Cell Populations
Experimental Detection and Visualization of the Extracellular Matrix in Macrocolony Biofilms
Congo Red Stain Identifies Matrix Overproduction and is an Indirect Measurement for c-di-GMP in many Species of Bacteria
Type IV pili-dependent Motility as a Tool to Determine the Activity of c-di-GMP Modulating Enzymes in Myxococcus xanthus
Using Light-Activated Enzymes for Modulating Intracellular c‑di-GMP Levels in Bacteria
Analysis of c-di-GMP Levels Synthesized by a Photoreceptor Protein in Response to Different Light Qualities using an in vitro Enzymatic Assay
Probing the Role of Cyclic di-GMP Signalling Systems in Disease using Chinese Radish
Contribution of Cyclic di-GMP in the Control of Type III and Type VI Secretion in Pseudomonas aeruginosa
Semi-quantitative Analysis of the Red, Dry, and Rough Colony Morphology of Salmonella enterica serovar Typhimurium and Escherichia coli using Congo Red
Fluorescent 2-aminopurine c-di-GMP and GpG analogs as PDE Probes
Measuring Cyclic Diguanylate (c-di-GMP)-specific Phosphodiesterase Activity using the MANT-c-di-GMP Assay
Determining Phosphodiesterase Activity (Radioactive Assay)
Determining Diguanylate Cyclase Activity (Radioactive Assay)
Detection of c-di-GMP-responsive DNA Binding
Use of Nonradiochemical DNAse Footprinting to Analyze c-di-GMP Modulation of DNA-binding Proteins
Detection of Cyclic di-GMP Binding Proteins Utilizing a Biotinylated Cyclic di-GMP Pulldown Assay
Probing Protein-protein Interactions with Genetically Encoded Photo-activatable Crosslinkers
Identification of c-di-AMP-Binding Proteins Using Magnetic Beads
Pulldown with a c-di-GMP-specific Capture Compound Coupled to Mass Spectrometry as a Powerful Tool to Identify Novel Effector Proteins
Identification of c-di-GMP-responsive Riboswitches
Isothermal Titration Calorimetry to Determine Apparent Dissociation Constants (Kd) and Stoichiometry of Interaction (n) of c-di-GMP Binding Proteins
Targeting c-di-GMP Signaling, Biofilm Formation and Bacterial Motility With Small Molecules
Discovering Selective Diguanylatecyclases Inhibitors: From PleD to Discrimination of the Active Site of cyclic-di-GMP Phosphodiesterases
High throughput Screening for Compounds that Modulate the Cellular c-di-GMP Level in Bacteria
Genetic Tools to Study c-di-GMP-dependent Signaling in Pseudomonas aeruginosa. .