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This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

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1. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments

   Douglas W. Houston

2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos
   Thao Nguyen, Timothy J. Mitchison, and Martin Wuhr

3. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos

   Michael D. Sheets

4. Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

   Alexander A. Tokmakov and Ken-Ichi Sato

5. Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis 

   Ken-ichi Sato and Alexander A. Tokmakov

6. Embryological and Genetic Manipulation of Chick Development

   Laura S. Gammill, Bridget Jacques-Fricke, and Julaine Roffers-Agarwal

7. Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos

   Ana Paula Azambuja and Marcos Simoes-Costa

8. Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens

   Triveni Menon and Sreelaja Nair

9. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq)

   Karoline Holler and Jan Philipp Junker

10. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos

   Clare E. Buckley

11. Ex Utero Culture and Imaging of Mouse Embryos

   Sonja Nowotschin, Vidur Garg, Anna Piliszek, and Anna-Katerina Hadjantonakis

12. Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

   Edwina McGlinn, Miriam Holzman, and Jennifer H. Mansfield

13. Reptile Embryology and Regeneration

   Matthew K. Vickaryous and Emily A.B. Gilbert

14. Manipulation of Developmental Function in Turtles with Notes on Alligators

   Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, and Judith A. Cebra-Thomas

15. Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes

   Amanda Butler, Dawn Owens, Mary Lou King, and Tristan Aguero

16. Visualizing the Balbiani Body in Zebrafish Oocytes

   KathyAnn L. Lee and Florence L. Marlow

17. Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis

   Allison Jamieson-Lucy and Mary C. Mullins

18. Generation of Xenopus Haploid, Triploid, and Hybrid Embryos

   Romain Gibeaux and Rebecca Heald

19. Germ Cell Transplantation in Avian Species

   Young Hyun Park, Young Min Kim, and Jae Yong Han

20. Production of Germ-Line Chimeras in Zebrafish

   Taiju Saito, Rie Goto, Nicola Rivers, and Etsuro Yamaha

21. A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring

   Zsolt Csenki and Ferenc Mueller

22. Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT)

   Sukumal Prukudom, Kannika Siripattarapravat, William Poulos, and Jose B. Cibelli

23. An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish

   Cara E. Moravec and Francisco J. Pelegri

24. Modeling Embryonic Cleavage Patterns

   Dmitry Ershov and Nicolas Minc

25. Laboratory Guidelines for Animal Care

   Marcelo Couto and Charles Cates

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