| 000 |
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03265camuu2200421 a 4500 |
| 001 |
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000000827564 |
| 005 |
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20060529095701 |
| 008 |
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940623s1994 nyua bf 001 0 eng |
| 010 |
|
▼a 94022457
|
| 020 |
|
▼a 0879693843
|
| 040 |
|
▼a DLC
▼c DLC
▼d COU
▼d TMV
▼d NLM
▼d AGL
▼d UBA
▼d 211009
|
| 049 |
1
|
▼l 131015190
▼f 의학
|
| 050 |
0
0
|
▼a QL737.R6
▼b M2468 1994
|
| 060 |
0
0
|
▼a 1995 C-524
|
| 060 |
1
0
|
▼a QY 25
▼b M278 1994
|
| 070 |
0
|
▼a QL737.R6M2468
▼b 1994
|
| 072 |
0
|
▼a L210
|
| 082 |
0
0
|
▼a 571.861935
▼2 22
|
| 090 |
|
▼a 571.861935
▼b M278-2
|
| 245 |
0
0
|
▼a Manipulating the mouse embryo :
▼b a laboratory manual /
▼c Brigid Hogan ... [et al.].
|
| 250 |
|
▼a 2nd ed.
|
| 260 |
|
▼a Plainview, N.Y. :
▼b Cold Spring Harbor Laboratory Press ,
▼c 1994.
|
| 300 |
|
▼a xvii, 497 p. :
▼b ill. (some col.) ;
▼c 28 cm.
|
| 504 |
|
▼a Includes bibliographical references (p. 439-470) and index.
|
| 505 |
0
|
▼a Developmental genetics and embryology of the mouse: past, present and future -- Summary of mouse development -- Setting up a colony for the production of transgenic and mutant mice -- Recovery, culture and transfer of embryos and germ cells -- In vitro manipulation of preimplantation embryos -- Production of transgenic mice -- Isolation, culture and manipulation of embryonic stem cells -- Analysis of transgenic mice -- Techniques for visualizing genes, gene products and specialized cell types.
|
| 505 |
0
|
▼a In vitro culture of eggs, embryos, primordial germ cells and teratocarcinoma cells -- Buffers and solutions -- Acidic tyrode solution for removing zonae -- Alkaline phosphatase buffers -- Alkaline phosphatase staining solution -- Avertin, anesthetic -- Bovine serum albumin (BSA) -- Ca??/Mg??-free PBS -- Ca??/Mg??-free tyrode ringer's saline (pH 7.6-7.7) -- Hyaluronidase -- NTM buffer (NTMT without tween 20) for in situ hybridization of intact embryos with RNA probes -- NTMT buffer for in situ hybridization of intact embryos with RNA probes.
|
| 505 |
0
|
▼a Pancreatin/trypsin solution for separating germ and tissue layers -- PBSMT for immunohistochemistry of whole mount embryos -- PBT -- Phenol/chloroform solution for isolating total RNA from mouse embryos or fetal tissues -- Phosphate-buffered saline (PBS @-- Pronase solution -- Saline/EDTA buffer plus glucose for isolation of germ cells and tissue culture -- 20X SSC -- 20X SSPE -- TE (tris/EDTA) buffer -- 0.25% trypsin in tris-saline for tissue culture -- Trypsin/EDTA solutions -- Sources of information -- Genetic resources -- Books -- Journals.
|
| 505 |
0
|
▼a Databases -- Software -- Suppliers -- Inbred, hybrid and random-bred mice -- Mouse genomic DNAs and RNAs -- Mouse genomic and cDNA libraries and clones -- Equipment and supplies.
|
| 650 |
0
|
▼a Mice
▼x Embryos
▼x Laboratory manuals.
|
| 650 |
0
|
▼a Mice
▼x Genetic engineering
▼x Laboratory manuals.
|
| 650 |
0
|
▼a Transgenic mice
▼x Laboratory manuals.
|
| 650 |
0
|
▼a Mice as laboratory animals.
|
| 650 |
1
2
|
▼a Mice
▼x embryology
▼v laboratory manuals.
|
| 650 |
1
2
|
▼a Genetic Engineering
▼v laboratory manuals.
|
| 650 |
2
2
|
▼a Mice, Transgenic
▼x genetics
▼v laboratory manuals.
|
| 650 |
7
|
▼a Animais de laboratorio
▼2 larpcal
|
| 700 |
1
|
▼a Hogan, Brigid.
|
