| 000 | 00000nam u2200205 a 4500 | |
| 001 | 000046092235 | |
| 005 | 20210913144003 | |
| 008 | 210913s2020 nyua b 001 0 eng d | |
| 020 | ▼a 9781071608012 | |
| 040 | ▼a 211009 ▼c 211009 ▼d 211009 | |
| 082 | 0 4 | ▼a 571.96 ▼2 23 |
| 084 | ▼a 571.96 ▼2 DDCK | |
| 090 | ▼a 571.96 ▼b I332 | |
| 245 | 0 0 | ▼a Immunometabolism : ▼b methods and protocols / ▼c edited by Suresh Mishra. |
| 260 | ▼a New York : ▼b Humana Press, ▼c c2020. | |
| 300 | ▼a xvi, 284 p. : ▼b ill. (some col.) ; ▼c 26 cm. | |
| 490 | 1 | ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 2184 |
| 490 | 1 | ▼a Springer protocols |
| 504 | ▼a Includes bibliographical references and index. | |
| 650 | 0 | ▼a Immunology ▼v Laboratory manuals. |
| 650 | 0 | ▼a Metabolism ▼v Laboratory manuals. |
| 700 | 1 | ▼a Mishra, Suresh. |
| 830 | 0 | ▼a Methods in molecular biology ; ▼v 2184. |
| 830 | 0 | ▼a Springer protocols. |
| 945 | ▼a KLPA |
소장정보
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 과학도서관/Sci-Info(2층서고)/ | 청구기호 571.96 I332 | 등록번호 521006082 | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
컨텐츠정보
책소개
This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism.
New feature
This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism.
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목차
1. Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq Janilyn Arsenio 2. Monitoring Lactate Dynamics in Individual Macrophages with a Genetically-Encoded Probe Felipe Baeza-Lehnert, Carlos A. Flores, Anita Guequen, and L. Felipe Barros 3. The Conjugation of Antibodies for the Simultaneous Detection of Surface Proteins and Transcriptome Analysis at a Single-Cell Level Iivari Kleino, Eliisa Kekalainen, and Tapio Loennberg 4. Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling Kevin J. Williams and Steven J. Bensinger 5. Proteomic Network Analysis of Polarized Macrophages Jayanta K. Chakrabarty, Abu Hena Mostafa Kamal, A.D.A. Shahinuzzaman, and Saiful M. Chowdhury 6. The Purification and Characterization of Exosomes from Macrophages Eran Fridman, Lana Ginini, Ziv Gil, and Neta Milman 7. The Isolation and Characterization of T Lymphocyte Exosomes Using Mass Spectrometry Inbar Azoulay-Alfaguter and Adam Mor 8. Analysis of Immune-Tumor Cell Interactions Using a 3D Co-Culture Model Tanya N. Augustine 9. Studying Adipocyte and Immune Cell Crosstalk Using a Co-Culture System Jennifer M. Monk, Danyelle M. Liddle, Amber L. Hutchinson, and Lindsay E. Robinson 10. A Gene Expression Analysis of M1 and M2 Polarized Macrophages Nour Eissa, Hayam Hussein, and Jean-Eric Ghia 11. Simultaneous, Quantitative Characterization of Protein ADP-Ribosylation and Protein Phosphorylation in Macrophages Casey M. Daniels, Arthur Nuccio, Pauline R. Kaplan, and Aleksandra Nita-Lazar 12. The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer Bridgette M. Cumming, Vineel P. Reddy, and Adrie J.C. Steyn 13. Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by Assessing Their Oxygen Consumption and Extracellular Acidification Hsi-Ju Wei, John J. Letterio, and Tej K. Pareek 14. The Evaluation of Mitochondrial Membrane Potential Using Fluorescent Dyes or a Membrane-Permeable Cation (TPP+) Electrode in Isolated Mitochondria and Intact Cells Joao S. Teodoro, Ivo F. Machado, Ana C. Castela, Anabela P. Rolo and Carlos M. Palmeira 15. The Multiparametric Analysis of Mitochondrial Dynamics in T Cells from Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) Jesse J.R. Masson, Matias Ostrowski, Gabriel Duette, Man kit Sam Lee, Andrew J. Murphy, Suzanne M. Crowe, and Clovis S. Palmer 16. The Measurement of Whole Body Glucose Homeostasis in Mice Yang Xin Zi Xu, Sudharsana R. Ande, and Suresh Mishra 17. Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy Andrew D. Sheppard and Joanne Lysaght 18. Sex Differences in Immunometabolism: An Unexplored Area Suresh Mishra, Geetika Bassi, and Yang Xin Zi Xu 19. Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis Nnamdi Ikeogu, Chidalu A. Edechi, Gloria Akaluka, Aida Feiz-Barazandeh, and Jude E. Uzonna