| 000 | 01023camuu2200301 a 4500 | |
| 001 | 000045669257 | |
| 005 | 20110930202641 | |
| 008 | 110930s2011 nyua b 001 0 eng d | |
| 015 | ▼a GBB156723 ▼2 bnb | |
| 020 | ▼a 9781617791840 (cased) | |
| 020 | ▼a 1617791849 (cased) | |
| 020 | ▼a 9781617791857 (e-book) | |
| 040 | ▼a StDuBDS ▼b eng ▼c StDuBDS ▼d Uk ▼d 211009 | |
| 082 | 0 4 | ▼a 571.6 ▼2 22 |
| 084 | ▼a 571.6 ▼2 DDCK | |
| 090 | ▼a 571.6 ▼b C615 | |
| 245 | 0 0 | ▼a Claudins : ▼b methods and protocols / ▼c edited by Kursad Turksen. |
| 260 | ▼a New York, N.Y. : ▼b Humana Press, ▼c c2011. | |
| 300 | ▼a xiv, 447 p. : ▼b ill. (some col.). ; ▼c 26 cm. | |
| 490 | 1 | ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 762 |
| 490 | 1 | ▼a Springer protocols |
| 504 | ▼a Includes bibliographical references and index. | |
| 650 | 0 | ▼a Tight junctions (Cell biology) |
| 700 | 1 | ▼a Turksen, Kursad. |
| 830 | 0 | ▼a Methods in molecular biology ; ▼v 762. |
| 830 | 0 | ▼a Springer protocols. |
| 945 | ▼a KLPA |
소장정보
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 과학도서관/Sci-Info(2층서고)/ | 청구기호 571.6 C615 | 등록번호 121213754 | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
컨텐츠정보
책소개
Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles.? In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins.? Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
?
Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.This volume in the Methods in Molecular Biology™ series collects techniques involving claudins and the opportunity they provide for understanding of the molecular basis of the tight junction proteins. Includes materials lists, protocols, tips and pitfalls.
New feature
Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles.? In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins.? Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
?
Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.정보제공 :
목차
1. Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling Christina M. Van Itallie and James M. Anderson 2. Biochemical Analysis of Claudin Binding Compatibility Christina Ward and Michael Koval 3. Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines Alan S.L. Yu 4. The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its Status Makoto Osanai 5. Interactions between Clostridium perfringens Enterotoxin and Claudins Susan L. Robertson and Bruce A. McClane 6. Biophysical Methods to Probe Claudin Mediated Adhesion at the Cellular and Molecular Level Sri Ram Krishna Vedula, Tong Seng Lim, Walter Hunziker, and Chwee Teck Lim 7. Detection of Tight Junction Barrier Function In Vivo by Biotin Lei Ding, Yuguo Zhang, Rodney Tatum, and Yan-Hua Chen 8. The Co-Culture Method to Examine Interactions between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells Tetsuichiro Inai 9. Claudin 4: Functional Studies Beyond the Tight Junction Holly A. Eckelhoefer, Thejani E. Rajapaksa, Jing Wang, Mary Stover-Hamer, Nancy C. Appleby, Jun Ling, and David D. Lo 10. Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin 16 P. Jaya Kausalya and Walter Hunziker 11. Claudin Family Proteins in C. elegans Jeffrey S.Simske and Jeff Hardin 12. In Vivo Imaging of Tight Junctions Using Claudin-EGFP Transgenic Medaka Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki 13. Claudins in a Primary Cultured Puffer Fish ( Tetraodon nigroviridis ) Gill Epithelium Phuong Bui and Scott P. Kelly 14. Manipulating Claudin Expression in Avian Embryos Michelle M. Collins and Aimee K. Ryan 15. Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues Lorenza Gonzalez-Mariscal, Erika Garay, and Miguel Quiros 16. Expression and Function of Claudins in Hepatocytes Takashi Kojima and Norimasa Sawada 17. Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Culture Junko Fujita-Yoshigaki 18. Development of Biological Tools to Study Claudins in the Male Reproductive Tract Daniel G. Cyr, Evemie Dube, Julie Dufresne, and Mary Gregory 19. Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo Julian A. Guttman 20. Laboratory Methods in the Study of Endometrial Claudin-4 Paulo Serafini, Andre Monteiro Rocha, Gary Daniel Smith, Eduardo Leme Alves da Motta, and Edmund Chada Baracat 21. Role of Claudins in Oxidant-Induced Alveolar Epithelial Barrier Dysfunction Yu Sun, Richard D. Minshall, and Guochang Hu 22. Tracing the Endocytosis of Claudin-5 in Brain Endothelial Cells Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic 23. Quantitative In Situ Analysis of Claudin Expression at the Blood-Retinal Barrier Heping Xu and Janet Liversidge 24. MMP-Mediated Disruption of Claudin-5 in the Blood-Brain Barrier of Rat Brain after Cerebral Ischemia Yi Yang and Gary A. Rosenberg 25. Claudin-5 Expression in In Vitro Models of the Blood-Brain Barrier Itzik Cooper, Katayun Cohen-Kashi-Malina, and Vivian I. Teichberg 26. HIV-1-Induced Alterations of Claudin-5 Expression at the Blood Brain Barrier Level Ibolya E. Andras and Michal Toborek 27. Enhanced Immunohistochemical Resolution of Claudin Proteins in Glycolmethacrylate-Embedded Tissue Biopsies Jane E. Collins, Adam Kirk, Sara K. Campbell, Juan Mason, and Susan J. Wilson 28. Claudin-16/Paracellin-1, Cloning, Expression, and Its Role in Tight Junction Functions in Cancer and Endothelial Cells Tracey A. Martin and Wen G. Jiang 29. Dynamics of Claudins Expression in Colitis and Colitis-Associated Cancer in Rat Yoshiaki Arimura, Kanna Nagaishi, a
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