| 000 | 00000cam u2200205 a 4500 | |
| 001 | 000045907311 | |
| 005 | 20170602161737 | |
| 008 | 170602s2017 nyua b 001 0 eng d | |
| 020 | ▼a 9781493968732 | |
| 020 | ▼a 1493968734 | |
| 029 | 0 | ▼a ZWZ ▼b 199466653 |
| 035 | ▼a (OCoLC)976169503 | |
| 040 | ▼a ZWZ ▼b fre ▼e ncafnor ▼c ZWZ ▼d OCLCQ ▼d KUB ▼d 211009 | |
| 049 | ▼a KUBA | |
| 060 | ▼a QU 25 | |
| 082 | 0 4 | ▼a 571.6 ▼2 23 |
| 084 | ▼a 571.6 ▼2 DDCK | |
| 090 | ▼a 571.6 ▼b C547 | |
| 245 | 0 0 | ▼a Cholesterol Homeostasis : ▼b methods and protocols / ▼c edited by Ingrid C. Gelissen, Andrew J. Brown. |
| 260 | ▼a New York : ▼b Humana Press : ▼b Springer, ▼c c2017. | |
| 300 | ▼a xi, 300 p. : ▼b ill. (some col.) ; ▼c 27 cm. | |
| 490 | 1 | ▼a Springer protocols |
| 490 | 1 | ▼a Methods in molecular biology, ▼x 1940-6029 ; ▼v 1583 |
| 504 | ▼a Includes bibliographical references and index. | |
| 700 | 1 | ▼a Gelissen, Ingrid C. |
| 700 | 1 | ▼a Brown, Andrew J. |
| 830 | 0 | ▼a Springer protocols. |
| 830 | 0 | ▼a Methods in molecular biology ; ▼v 1583. |
| 945 | ▼a KLPA |
소장정보
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 과학도서관/Sci-Info(2층서고)/ | 청구기호 571.6 C547 | 등록번호 121240579 | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
컨텐츠정보
책소개
1. An Overview of Cholesterol Homeostasis
Ingrid C. Gelissen and Andrew J. Brown
2. Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol Binding Domains in Membrane Proteins
Coralie Di Scala and Jacques Fantini
3. Structural Stringency of Cholesterol for Membrane Protein Function utilizing Stereoisomers as Novel Tools: A Review
Md. Jafurulla and Amitabha Chattopadhyay
4. Manipulating Cholesterol Status within Cells
Winnie Luu, Ingrid C. Gelissen, and Andrew J. Brown
5. Assaying Low-Density-Lipoprotein (LDL) Uptake into CellsAnke Loregger, Jessica Kristine Nelson, and Noam Zelcer
6. The use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolaemia and Atherosclerosis
Eser J. Zerenturk and Anna C. Calkin
7. CRISPR/Cas9-mediated Generation of Niemann-Pick C1 Knockout Cell Line
Ximing Du, Ivan Lukmantara, Hongyuan Yang
8. Quantitative Measurement of Cholesterol in Cell Population Using Flow Cytometry and Fluorescent Perfringolysin O*Jian Li, Peter L. Lee, and Suzanne R. Pfeffe
9. Transport Assays for Sterol-binding Proteins: Stopped-flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein
Leslie A McCauliff1 and Judith Storch
10. Synthesis and Live-cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport
Maciej Modzel, Frederik W. Lund, and Daniel W?stner
11. Measurement of Cholesterol Transfer from Lysosome to Peroxisomes Using an In Vitro Reconstitution Assay
Jie Luo, Ya-Cheng Liao, Jian Xiao, and Bao-Liang Song
12. Measurement of Mitochondrial Cholesterol Import using a Mitochondria-targeted CYP11A1 Fusion Construct
Barry Edward Kennedy, Mark Charman, and Barbara Karten
13. Identifying Sterol Response Elements within Promoters of Genes
Laura J. Sharpe and Andrew J. Brown
14. Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion
Lindsey L. Morris and Russell A. DeBose-Boyd
15. Determining the Topology of Membrane-bound Proteins using PEGylation
Vicky Howeand Andrew J Brown
16. Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry
Anika V. Prabhu, Winnie Luu, and Andrew J. Brown
17. Sterol Analysis by Quantitative Mass Spectrometry
Andrew M. Jenner and Simon H.J. Brown
18. Measurement of Rates of Cholesterol and Fatty Acid Synthesis in vivo using Tritiated Water
Adam M. Lopez, Jen-Chieh Chuang and Stephen D. Turley
19. Methods for Monitoring ABCA1-dependent Sterol Release
Yoshio Yamauchi, Shinji Yokoyama, and Ta-Yuan Chang
20. ABC-transporter Mediated Sterol Export from Cells using Radio-Labeled Sterols
Alryel Yang and Ingrid C. Gelissen
21. Measurement of Macrophage-specific In Vivo Reverse Cholesterol Transport in Mice
M. Kockx, W. Jessup and L. Kritharides
정보제공 :
목차
An Overview of Cholesterol Homeostasis Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol Binding Domains in Membrane Proteins Structural Stringency of Cholesterol for Membrane Protein Function utilizing Stereoisomers as Novel Tools: A Review Manipulating Cholesterol Status within Cells Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells The use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolaemia and Atherosclerosis CRISPR/Cas9-mediated Generation of Niemann-Pick C1 Knockout Cell Line Quantitative Measurement of Cholesterol in Cell Population Using Flow Cytometry and Fluorescent Perfringolysin O* Transport Assays for Sterol-binding Proteins: Stopped-flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein Synthesis and Live-cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport Measurement of Cholesterol Transfer from Lysosome to Peroxisomes Using an In Vitro Reconstitution Assay Measurement of Mitochondrial Cholesterol Import using a Mitochondria-targeted CYP11A1 Fusion Construct Identifying Sterol Response Elements within Promoters of Genes Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion Determining the Topology of Membrane-bound Proteins using PEGylation Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry Sterol Analysis by Quantitative Mass Spectrometry Measurement of Rates of Cholesterol and Fatty Acid Synthesis in vivo using Tritiated Water Methods for Monitoring ABCA1-dependent Sterol Release ABC-transporter Mediated Sterol Export from Cells using Radio-Labeled Sterols Measurement of Macrophage-specific In Vivo Reverse Cholesterol Transport in Mice.