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Cell viability assays : methods and protocols

Cell viability assays : methods and protocols (2회 대출)

자료유형
단행본
개인저자
Gilbert, Daniel F. Friedrich, Oliver.
서명 / 저자사항
Cell viability assays : methods and protocols / edited by Daniel F. Gilbert, Oliver Friedrich.
발행사항
New York :   Humana Press :   Springer,   c2017.  
형태사항
xii, 299 p. : ill. (some col.) ; 27 cm.
총서사항
Methods in molecular biology,1940-6029 ; 1601
ISBN
9781493969593 (print) 9781493969609 (electronic bk.) 1493969609 (electronic bk.)
요약
This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.
내용주기
Basic colorimetric proliferation assays : MTT, WST, and resazurin / Konstantin Präbst, Hannes Engelhardt, Stefan Ringgeler, and Holger Hübner -- Assaying cellular viability using the neutral red uptake assay / Gamze Ates, Tamara Vanhaecke, Vera Rogiers, and Robim M. Rodrigues -- Assessment of cell viability with single-, dual-, and multi-staining methods using image cytometry / Leo Li-Ying Chan, Kelsey J. McCulley, and Sarah L. Kessel -- High-throughput spheroid screens using volume, resazurin reduction, and acid phosphatase Activity / Delyan P. Ivanov, Anna M. Grabowska, and Martin C. Garnett -- Protocol for in vitro high-throughput chemical susceptibility screening in differentiating NT2 stem cells / Ann-Katrin Menzner and Daniel F. Gilbert -- Ferroptosis and cell death analysis by flow cytometry / Daishi Chen, Ilker Y. Eyupoglu, and Nicolai Savaskan -- Assaying mitochondrial respiration as an indicator of cellular metabolism and fitness / Natalia Smolina, Joseph Bruton, Anna Kostareva, and Thomas Sejersen -- ATP-based luciferase viability assay for animal african trypanosomes using a 96-well plate / Keisuke Suganuma, Nthatisi Innocentia Molefe, and Noboru Inoue -- SYBR® green I-based fluorescence assay to assess cell viability of malaria parasites for routine use in compound sreening / Maria Leidenberger, Cornelia Voigtländer, Nina Simon, and Barbara Kappes -- Screening applications to test cellular fitness in Transwell® models after nanoparticle treatment / Bastian Christ, Christina Fey, Alevtina Cubukova, Heike Walles, Sofia Dembski, and Marco Metzger -- Assays for analyzing the role of transport proteins in the uptake and the vectorial transport of substances affecting cell viability / Emir Taghikhani, Martin F. Fromm, and Jörg König -- Metabolite profiling of mammalian cell culture processes to evaluate cellular viability / Isobelle M. Evie, Alan J. Dickson, and Mark Elvin -- Assaying spontaneous network activity and cellular viability -- Using multi-well microelectrode arrays / Jasmine P. Brown, Brittany S. Lynch, Itaevia M. Curry-Chisolm, Timothy J. Shafer, and Jenna D. Strickland -- Quantitative ratiometric Ca2+ imaging to assess cell viability / Oliver Friedrich and Stewart I. Head -- Functional viability : measurement of synaptic vesicle pool sizes / Jana K. Wrosch and Teja W. Groemer -- Phenotyping cellular viability by functional analysis of ion channels : GlyR-targeted screening in NT2-N cells / Katharina Kuenzel, Sepideh Abolpour Mofrad, and Daniel F. Gilbert -- Systematic cell-based phenotyping of missense alleles / Aenne S. Thormählen and Heiko Runz -- Second harmonic generation microscopy of muscle cell morphology and dynamics / Andreas Buttgereit -- Assessment of population and ECM production using multiphoton microscopy as an indicator of cell viability / Martin Vielreicher and Oliver Friedrich -- Average rheological quantities of cells in monolayers / Haider Dakhil and Andreas Wierschem -- Measurement of cellular behavior by electrochemical impedance sensing / Simin Öz, Achim Breiling, and Christian Maercker -- Nano-QSAR model for predicting cell viability of human embryonic kidney cells / Serena Manganelli and Emilio Benfenati.
서지주기
Includes bibliographical references and index.
일반주제명
Cytology --Laboratory manuals. Cell Survival.
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245 0 0 ▼a Cell viability assays : ▼b methods and protocols / ▼c edited by Daniel F. Gilbert, Oliver Friedrich.
260 ▼a New York : ▼b Humana Press : ▼b Springer, ▼c c2017.
300 ▼a xii, 299 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Methods in molecular biology, ▼x 1940-6029 ; ▼v 1601
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
505 0 0 ▼t Basic colorimetric proliferation assays : MTT, WST, and resazurin / ▼r Konstantin Präbst, Hannes Engelhardt, Stefan Ringgeler, and Holger Hübner -- ▼t Assaying cellular viability using the neutral red uptake assay / ▼r Gamze Ates, Tamara Vanhaecke, Vera Rogiers, and Robim M. Rodrigues -- ▼t Assessment of cell viability with single-, dual-, and multi-staining methods using image cytometry / ▼r Leo Li-Ying Chan, Kelsey J. McCulley, and Sarah L. Kessel -- ▼t High-throughput spheroid screens using volume, resazurin reduction, and acid phosphatase Activity / ▼r Delyan P. Ivanov, Anna M. Grabowska, and Martin C. Garnett -- ▼t Protocol for in vitro high-throughput chemical susceptibility screening in differentiating NT2 stem cells / ▼r Ann-Katrin Menzner and Daniel F. Gilbert -- ▼t Ferroptosis and cell death analysis by flow cytometry / ▼r Daishi Chen, Ilker Y. Eyupoglu, and Nicolai Savaskan -- ▼t Assaying mitochondrial respiration as an indicator of cellular metabolism and fitness / ▼r Natalia Smolina, Joseph Bruton, Anna Kostareva, and Thomas Sejersen -- ▼t ATP-based luciferase viability assay for animal african trypanosomes using a 96-well plate / ▼r Keisuke Suganuma, Nthatisi Innocentia Molefe, and Noboru Inoue -- ▼t SYBR® green I-based fluorescence assay to assess cell viability of malaria parasites for routine use in compound sreening / ▼r Maria Leidenberger, Cornelia Voigtländer, Nina Simon, and Barbara Kappes -- ▼t Screening applications to test cellular fitness in Transwell® models after nanoparticle treatment / ▼r Bastian Christ, Christina Fey, Alevtina Cubukova, Heike Walles, Sofia Dembski, and Marco Metzger -- ▼t Assays for analyzing the role of transport proteins in the uptake and the vectorial transport of substances affecting cell viability / ▼r Emir Taghikhani, Martin F. Fromm, and Jörg König -- ▼t Metabolite profiling of mammalian cell culture processes to evaluate cellular viability / ▼r Isobelle M. Evie, Alan J. Dickson, and Mark Elvin -- ▼t Assaying spontaneous network activity and cellular viability -- ▼t Using multi-well microelectrode arrays / ▼r Jasmine P. Brown, Brittany S. Lynch, Itaevia M. Curry-Chisolm, Timothy J. Shafer, and Jenna D. Strickland -- ▼t Quantitative ratiometric Ca2+ imaging to assess cell viability / ▼r Oliver Friedrich and Stewart I. Head -- ▼t Functional viability : measurement of synaptic vesicle pool sizes / ▼r Jana K. Wrosch and Teja W. Groemer -- ▼t Phenotyping cellular viability by functional analysis of ion channels : GlyR-targeted screening in NT2-N cells / ▼r Katharina Kuenzel, Sepideh Abolpour Mofrad, and Daniel F. Gilbert -- ▼t Systematic cell-based phenotyping of missense alleles / ▼r Aenne S. Thormählen and Heiko Runz -- ▼t Second harmonic generation microscopy of muscle cell morphology and dynamics / ▼r Andreas Buttgereit -- ▼t Assessment of population and ECM production using multiphoton microscopy as an indicator of cell viability / ▼r Martin Vielreicher and Oliver Friedrich -- ▼t Average rheological quantities of cells in monolayers / ▼r Haider Dakhil and Andreas Wierschem -- ▼t Measurement of cellular behavior by electrochemical impedance sensing / ▼r Simin Öz, Achim Breiling, and Christian Maercker -- ▼t Nano-QSAR model for predicting cell viability of human embryonic kidney cells / ▼r Serena Manganelli and Emilio Benfenati.
520 ▼a This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.
650 0 ▼a Cytology ▼v Laboratory manuals.
650 2 ▼a Cell Survival.
655 2 ▼a Laboratory Manuals.
700 1 ▼a Gilbert, Daniel F.
700 1 ▼a Friedrich, Oliver.
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 1601.
830 0 ▼a Springer protocols.
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 571.6 C39396 등록번호 121240842 (2회 대출) 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

책소개

This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.



New feature

This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.




정보제공 : Aladin

저자소개

대니얼 길버트(엮은이)

하버드 대학교 심리학과 교수로 인간이 행복에 대해 어떻게 생각하는가를 수년간 연구했다. 강의와 연구 업적으로 구겐하임 연구비를 지원받았고, 심리학의 발전에 끼친 공로를 인정받아 미국심리학회의 우수과학업적상을 수상했다.

Oliver Friedrich(엮은이)

정보제공 : Aladin

목차

Basic Colorimetric Proliferation Assays: MTT, WST and Resazurin
Assaying Cellular Viability using the Neutral Red Uptake Assay
Assessment of Cell Viability with Single, Dual, and Multi-Staining Methods using Image Cytometry
High-throughput Spheroid Screens using Volume, Resazurin Reduction, and Acid Phosphatase Activity
A Protocol for in vitro High-throughput Chemical Susceptibility Screening in Differentiating NT2 Stem Cells
Ferroptosis and Cell Death Analysis by Flow Cytometry
Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness
An ATP-based Luciferase Viability Assay for Animal African Trypanosomes using a 96-well Plate
SYBRâ Green I Based Fluorescence Assay to Assess Cell Viability of Malaria Parasites for Routine Use in Compound Screening
Screening Applications to Test Cellular Fitness in Transwell® Models after Nanoparticle Treatment
Assays for Analyzing the Role of Transport Proteins in the Uptake and the Vectorial Transport of Substances Affecting Cell Viability
Metabolite Profiling Of Mammalian Cell Culture Processes to Evaluate Cellular Viability
Assaying Spontaneous Network Activity and Cellular Viability using Multi-well Microelectrode Arrays
Quantitative Ratiometric Ca2+ Imaging to Assess Cell Viability
Functional Viability: Measurement of Synaptic Vesicle Pool Sizes
Phenotyping Cellular Viability by Functional Analysis of Ion Channels: GlyR-targeted Screening in NT2-N Cells
Systematic Cell-Based Phenotyping of Missense Alleles
Second Harmonic Generation Microscopy of Muscle Cell Morphology and Dynamics
Assessment of Population and ECM Production Using Multiphoton Microscopy as an Indicator of Cell Viability
Average Rheological Quantities of Cells in Monolayers
Measurement of Cellular Behavior by Electrochemical Impedance Sensing
Nano-QSAR Model for Predicting Cell Viability of Human Embryonic Kidney Cells.

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