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Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.

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Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational for the study of tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis, and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

?Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves? as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.

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PART I IMAGING MORPHOGENESIS

 Chapter 1. Probing Regional Mechanical Properties of Embryonic Tissue Using Microindentation and Optical Coherence Tomography

Benjamin A. Filas, Gang Xu, and Larry A. Taber

Chapter 2. Hemodynamic Flow Visualization of Early Embryonic Great Vessels using mPIV

Selda Goktas, Chia-Yuan Chen, William J. Kowalski, and Kerem Pekkan

Chapter 3. Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis

Jacky G. Goetz, Fabien Monduc, Yannick Schwab, and Julien Vermot

Chapter 4. Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis

Chelsea L. Gregg, Andrew K. Recknagel, and Jonathan T. Butcher

Chapter 5. Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila Melanogaster Embryos

Sophia N. Carrell and Gregory T. Reeves

Chapter 6. Light-Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly

Khaled Khairy, William Lemon, Fernando Amat, and Philipp J. Keller

Chapter 7. Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis

Chun Yin Bosco Leung and Rodrigo Fernandez-Gonzalez

Chapter 8. A Multiplex Fluorescent in situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis

Lily S. Cheung and Stanislav Shvartsman

Chapter 9. Active Cell and ECM Movements During Development

Anastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, and Andras Czirok

PART II CULTURE MODELS OF MORPHOGENESIS

 Chapter 10. 3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion

Kim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner, Jennifer N. Beck, Kevin J. Cheung, and Andrew J. Ewald

Chapter 11. Culture of Mouse Embryonic Foregut Explants

Felicia Chen and Wellington V. Cardoso

Chapter 12. Investigating Human Vascular Tube Morphogenesis and maturation using endothelial cell-pericyte co-cultures and a doxycycline-inducible genetic system in 3D extracellular matrices

Stephanie L.K. Bowers, Chun-Xia Meng, Matthew T. Davis, and George E. Davis

Chapter 13. Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues

Alexandra S. Piotrowski, Victor D. Varner, Nikolce Gjorevski, and Celeste M. Nelson

PART III MANIPULATING CELLS AND TISSUES IN VIVO

Chapter 14. Probing Cell Mechanics with Sub-Cellular Laser Dissection of Actomyosin Networks in the Early Developing Drosophila Embryo

M. Rauzi and P.-F. Lenne

Chapter 15. UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in vivo and ex vivo

Michael Smutny, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp Heisenberg

Chapter 16. Measurement of Intercellular Cohesion by Tissue Surface Tension

Ramsey A. Foty

Chapter 17. Quail-Chick Chimeras and Eye Development

Sinu Jasrapuria Agrawal and Peter Y. Lwigale

PART IV EMERGING MODELS OF TISSUE MORPHOGENESIS

Chapter 18. Studying Epithelial Morphogenesis in Dictyostelium

Daniel J. Dickinson, W. James Nelson, and William I. Weis

Chapter 19. Primary Cell Cultures of Regenerating Holothurian Tissues

Samir A. Bello, Ricardo J. Abreu-Irizarry, and Jose E. Garcia-Arraras

PART V COMPUTATIONAL MODELS OF TISSUE MORPHOGENESIS

 Chapter 20. Large-Scale Parameter Studies of Cell-Based Models of Tissue Morphogenesis Using CompuCell3D or VirtualLeaf

Margriet M. Palm and Roeland M. H. Merks

Chapter 21. Simulating Tissue Morphogenesis and Signaling

Dagmar Iber, Simon Tanaka, Patrick Fried, Philipp Germann, and Denis Menshykau

Chapter 22. Elasticity-Based Targeted Growth Models of Morphogenesis

Patrick W. Alford

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