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Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Because there are already many manuals and protocols available, along with commercial kits and reagents, a variety of the more common techniques have not been included in our book. The protocols described, based on rather sophisticated techniques for in vivo and in vitro studies, consist of molecular biology, biochemistry, and various types of immunoassays. Indeed, the authors have successfully accomplished an arduous task by presenting several topics in the simplest possible manner. We are confident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle. We greatly acknowledge the extraordinary contribution of the authors in writing this book.

Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Because there are already many manuals and protocols available, along with commercial kits and reagents, a variety of the more common techniques have not been included in our book. The protocols described, based on rather sophisticated techniques for in vivo and in vitro studies, consist of molecular biology, biochemistry, and various types of immunoassays. Indeed, the authors have successfully accomplished an arduous task by presenting several topics in the simplest possible manner. We are confident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle. We greatly acknowledge the extraordinary contribution of the authors in writing this book.

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Part I. Analysis of Cyclins and Cyclin-Dependent Kinases
The Biology of Cyclins and Cyclin-Dependent Protein Kinases: An Introduction
Lucio Miele
In Situ Immunofluorescence Analysis: Immunofluorescence Microscopy
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein
In Situ Immunofluorescence Analysis: Analyzing RNA Synthesis by 5-Bromouridine-5'-Triphosphate Labeling
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein
Immunofluorescence Analysis Using Epitope-Tagged Proteins: In Vitro System
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein
Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein
Chromatin Immunoprecipitation
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Electrophoretic Mobility Shift Assay
AmjadJaved, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I Digestion
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Ligation-Mediated Polymerase Chain Reaction
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein
Assays for Cyclin-Dependent Kinase Inhibitors
Adrian M. Senderowicz. Protein Degradation Via the Proteosome, Henry Hoff, Hong Zhang, and Christian Sell
Part II. Analysis of the Factors Involved in Cell Cycle Deregulation
The Transformed Phenotype
Henry Hoff, Barbara Belletti, Hong Zhang, and Christian Sell
A Morphologic Approach to Detect Apoptosis Based on Electron Microscopy
Martyn K. White and Caterina Cinti
Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation
Carmela Trimarchi, Dario La Sala, Alessandra Zamparelli, and Caterina Cinti
Part III. Cellular Response to Deoxyribonucleic Acid Damage
Induction of Deoxyribonucleic Acid Damage by Alkylating Agents
Salvatore Cortellino, David P. Turner, Domenico Albino, and Alfonso Bellacosa
Induction of Deoxyribonucleic Acid Damage by g Irradiation
Salvatore Cortellino, David P. Turner and Alfonso

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