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A collection of new reviews and protocols from leading experts in cell cycle regulation, Cell Cycle Control: Mechanisms and Protocols, Second Edition presents a comprehensive guide to recent technical and theoretical advancements in the field. Beginning with the overviews of various cell cycle regulations, this title presents the most current protocols and state-of-the-art techniques used to generate latest findings in cell cycle regulation, such as protocols to analyze cell cycle events and molecules. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Cell Cycle Control: Mechanisms and Protocols, Second Edition will be a valuable resource for a wide audience, ranging from the experienced cell cycle researchers looking for new approaches to the junior graduate students giving their first steps in cell cycle research.

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A collection of new reviews and protocols from leading experts in cell cycle regulation, Cell Cycle Control: Mechanisms and Protocols, Second Edition presents a comprehensive guide to recent technical and theoretical advancements in the field. Beginning with the overviews of various cell cycle regulations, this title presents the most current protocols and state-of-the-art techniques used to generate latest findings in cell cycle regulation, such as protocols to analyze cell cycle events and molecules. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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Authoritative and easily accessible, Cell Cycle Control: Mechanisms and Protocols, Second Edition will be a valuable resource for a wide audience, ranging from the experienced cell cycle researchers looking for new approaches to the junior graduate students giving their first steps in cell cycle research.

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'Part I. Reviews: Cell Cycle Regulations

1. Cell Cycle-Regulated Transcription: Effectively Using a Genomics Toolbox
Sara L. Bristow, Adam R. Leman, and Steven B. Haase

2. Cell Cycle Regulation by Checkpoints
Kevin J. Barnum and Matthew O'Connell

3. Interplay Between the Cell Cycle and Double-Strand Break Response in Mammalian Cells

Kate Beishline and Jane Azizkhan-Clifford

4. Cell Cycle Regulation by Protein Degradation
Deana M. Koepp

5. Linking Chromosome Duplication to Segregation Via Sister Chromatid Cohesion
Adam R. Leman and Eishi Noguchi

6. The Greatwall-PP2A Axis in Cell Cycle Control
Peng Wang, Marcos Malumbres, and Vincent Archambault

7. Cell Cycle Regulation by the Nutrient-Sensing Mammalian Target of Rapamycin (mTOR) Pathway

Elisabet Cuyas, Bruna Corominas-Faja, Jorge Joven, and Javier A. Menendez

8. The Senescence Arrest Program and the Cell Cycle

Alessandro Bitto, Elizabeth Crowe, Chad Lerner, Claudio Torres, and Christian Sell

9. Oncogenic Ras Pushes (and Pulls) Cell Cycle Progression Through ERK Activation

Paul M. Campbell

10. Cell Cycle Regulation During Viral Infection
Sumedha Bagga and Michael J. Bouchard

11. The Roles of Cohesins in Mitosis, Meiosis, and Human Health and Disease
Amanda S. Brooker and Karen M. Berkowitz

12. Introductory Review of Computational Cell Cycle Modeling
Andres Kriete, Eishi Noguchi, and Christian Sell

Part II. Protocols: Analyzing Cell Cycle Regulations

13. Cell Cycle Synchronization and Flow Cytometry Analysis of Mammalian Cells

Naoko Yoshizawa-Sugata and Hisao Masai

14. Analyzing Transcription Dynamics During the Budding Yeast Cell Cycle
Adam R. Leman, Sara L. Bristow, and Steven B. Haase

15. Analyzing Cell Cycle Checkpoints in Response to Ionizing Radiation in Mammalian Cells

Bin Wang

16. Analyzing DNA Replication Checkpoint in Budding Yeast
Nicole Hustedt and Kenji Shimada

17. Analyzing Cell Cycle-Dependent Degradation and Ubiquitination in Budding Yeast

Dong-Hwan Kim and Deanna M. Koepp

18. Imaging Analysis of Cell Cycle-Dependent Degradation of Cdt1 in Mammalian Cells

Yasushi Shiomi, Naohiro Suenaga, Miyuki Tanaka, Akiyo Hayashi, and Hideo Nishitani

19. PCNA-Dependent Ubiquitination of Cdt1 and p21 in Mammalian Cells
Akiyo Hayashi, Naohiro Suenaga, Yasushi Shiomi, and Hideo Nishitani

20. Analyzing Cdc2/Cdk1 Activation During Stress Response in Schizosaccharomyces pombe

Miguel A. Rodriguez-Gabriel

21. Analyzing Ras-Associated Cell Proliferation Signaling

Matthew C. Stout, Edgar Asiimwe, James R. Birkenstamm, Su Yeon Kim and Paul M. Campbell

22. Telomere Regulation During Cell Cycle in Fission Yeast
Bettina A. Moser, Ya-Ting Chang, and Toru M. Nakamura

23. Detecting Senescence: Methods and Approaches

Elizabeth P. Crowe, Timothy Nacarelli, Alessandro Bitto, Chad Lerner, Christian Sell, and Claudio Torres

Part III. Protocols: Analyzing Cell Cycle Events and Molecules

24. Analyzing RB and E2F During the G1/S Transition
Michael J. Thwaites, Matthew J. Cecchini, and Frederick A. Dick

25. Analyzing G1/S Transcriptional Controls
Steffi Klier, Sarah Farmer, and Robertus A. M. de Bruin

26. Analysis of Replication Timing Using Synchronized Budding Yeast Cultures
Jie Peng, M. K. Raghuraman, and Wenyi Feng

27. Analysis of ssDNA Gaps and DSBs in Genetically Unstable Yeast Cultures
Jie Peng, M. K. Raghuraman, and Wenyi Feng

28. Chromatin Fractionation Analysis of Licensing Factors in Mammalian Cells

Hideo Nishitani, Masayuki Morino, Yusuke Murakami, Takeshi Maeda, and Yasushi Shiomi

29. Imaging Analysis to Determine Chromatin Binding of Licensing Factors MCM2-7 in Mammalian Cells

Mayayuki Morino, Miyuki Tanaka, Yasuhi Shiomi, and Hideo Nishitani

30. Chromatin Immunoprecipitation to Investigate Origin Association of Replication Factors in Mammalian Cells

Adam R. Leman and Eishi Noguchi

31. Live Cell Fluorescence Imaging for Phenotypic Ana

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