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Cell cycle oscillators : methods and protocols / 2nd ed

Cell cycle oscillators : methods and protocols / 2nd ed

자료유형
단행본
개인저자
Coutts, Amanda S. Weston, Louise.
서명 / 저자사항
Cell cycle oscillators : methods and protocols / edited by Amanda S. Coutts, Louise Weston.
판사항
2nd ed.
발행사항
New York :   Humana Press :   Springer,   c2021.  
형태사항
xii, 340 p. : ill. (some col.) ; 27 cm.
총서사항
Springer protocols
ISBN
9781071615379
서지주기
Includes bibliographical references and index.
일반주제명
Cell cycle --Laboratory manuals. Cell Cycle --Laboratory Manuals.
000 00000nam u2200205 a 4500
001 000046093799
005 20210928142255
008 210928s2021 nyua b 001 0 eng d
020 ▼a 9781071615379
040 ▼a 211009 ▼c 211009 ▼d 211009
082 0 4 ▼a 571.8/4 ▼2 23
084 ▼a 571.84 ▼2 DDCK
090 ▼a 571.84 ▼b C3937-2
245 0 0 ▼a Cell cycle oscillators : ▼b methods and protocols / ▼c edited by Amanda S. Coutts, Louise Weston.
250 ▼a 2nd ed.
260 ▼a New York : ▼b Humana Press : ▼b Springer, ▼c c2021.
300 ▼a xii, 340 p. : ▼b ill. (some col.) ; ▼c 27 cm.
490 1 ▼a Springer protocols
490 1 ▼a Methods in molecular biology ; ▼v 2329
504 ▼a Includes bibliographical references and index.
650 0 ▼a Cell cycle ▼v Laboratory manuals.
650 2 ▼a Cell Cycle ▼v Laboratory Manuals.
700 1 ▼a Coutts, Amanda S.
700 1 ▼a Weston, Louise.
830 0 ▼a Springer protocols.
830 0 ▼a Methods in molecular biology ; ▼v 2329.
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 571.84 C3937-2 등록번호 521006114 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

책소개

1. Cell Cycle Control: A System of Interlinking Oscillators

Randy Y.C. Poon

2. Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity

Matthew P. Swaffer

3. Real Time Monitoring of APC/C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts

Julia Kamenz, Renping Qiao, Qiong Yang, and James E. Ferrell, Jr.

4. Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts

Morgan Pellerano and May C. Morris

5. Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry

Andrew G. DeMarco, Pete E. Pascuzzi, W. Andy Tao, and Mark C. Hall

6. Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots

Hirotomo Takatsuka and Masaaki Umeda

7. Construction of a Full-Length 3'UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs

Dominika Kazmierczak and Per Hydbring

8. Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis

Mardo K?ivom?gi

9. Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block

Arif A. Surani, Sergio L. Colombo, George Barlow, Gemma A. Foulds, and Cristina Montiel-Duarte

10. Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition

Pawan Singh, Lenka Halova, and Iain Michael Hagan

11. Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System

Danit Wasserman, Sapir Nachum, Meirav Noach-Hirsh, Naomi Auerbach, Evelin Sheinberger-Chorni, Taylor P Enrico, Roxane Lahmi, Michael J. Emanuele, and Amit Tzur

12. EDU (5-Ethynyl-2′-Deoxyuridine)-Coupled-Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle

Jo?o A. Ferreira, Marco Neves, Miguel Alpalh?o, Pedro Pereira, Daniela Cunha, Fernando Ferreira, Ren? Santus, Ana E. Sousa, and Paulo L. Filipe

13. High-Resolution Analysis of Centrosome Behavior During Mitosis

Vanessa Nunes, Margarida Dantas, Joana T. Lima, and Jorge G. Ferreira

14. Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells

Jonathan M. Geisinger and Tim Stearns

15. Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle

Isha Nasa, Greg B. Moorhead, and Arminja N. Kettenbach

16. Visualization of Radiation-Induced Cell Cycl


정보제공 : Aladin

목차

1. Cell Cycle Control: A System of Interlinking Oscillators



Randy Y.C. Poon







2. Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity



Matthew P. Swaffer







3. Real Time Monitoring of APC/C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts



Julia Kamenz, Renping Qiao, Qiong Yang, and James E. Ferrell, Jr.







4. Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts



Morgan Pellerano and May C. Morris







5. Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry



Andrew G. DeMarco, Pete E. Pascuzzi, W. Andy Tao, and Mark C. Hall







6. Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots



Hirotomo Takatsuka and Masaaki Umeda







7. Construction of a Full-Length 3''UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs



Dominika Kazmierczak and Per Hydbring







8. Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis



Mardo Koivomagi







9. Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block



Arif A. Surani, Sergio L. Colombo, George Barlow, Gemma A. Foulds, and Cristina Montiel-Duarte







10. Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition



Pawan Singh, Lenka Halova, and Iain Michael Hagan







11. Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System



Danit Wasserman, Sapir Nachum, Meirav Noach-Hirsh, Naomi Auerbach, Evelin Sheinberger-Chorni, Taylor P Enrico, Roxane Lahmi, Michael J. Emanuele, and Amit Tzur







12. EDU (5-Ethynyl-2''-Deoxyuridine)-Coupled-Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle



Joao A. Ferreira, Marco Neves, Miguel Alpalhao, Pedro Pereira, Daniela Cunha, Fernando Ferreira, Rene Santus, Ana E. Sousa, and Paulo L. Filipe







13. High-Resolution Analysis of Centrosome Behavior During Mitosis



Vanessa Nunes, Margarida Dantas, Joana T. Lima, and Jorge G. Ferreira







14. Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells



Jonathan M. Geisinger and Tim Stearns







15. Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle



Isha Nasa, Greg B. Moorhead, and Arminja N. Kettenbach







16. Visualization of Radiation-Induced Cell Cycle Kinetics with a Fluorescent Ubiquitination-Based Cell Cycle Indicator (Fucci)



Atsushi Kaida and Masahiko Miura







17. Dynamic Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody



Yuko Sato and Hiroshi Kimura







18. Analyzing Centrioles and Cilia by Expansion Microscopy



Dong Kong and Jadranka Loncarek







19. Analysis of Cell Cycle Progression in the Budding Yeast S. cerevisiae



Deniz Pirincci Ercan and Frank Uhlmann







20. Application of PALM Super Resolution Microscopy to the Analysis of Microtubule-Organizing Centers (MTOCs) in Aspergillus nidulans



Xiaolei Gao, Reinhard Fischer, and Norio Takeshita







21. Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools



Melinda Van Kerckvoorde, Matthew J. Ford, Patricia L. Yeyati, Pleasantine Mill, and Richard L. Mort







22. Calorimetric Heat Dissipation Measurements of Developing Zebrafish Embryos



Jonathan Rodenfels and Karla M. Neugebauer







23. The Conditional Knockout Analogous System: CRISPR-Mediated Knock Out Together with Inducible Degron and Transcription-Controlled Expression



Hoi Tang Ma

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