| 000 | 00000cam u2200205 a 4500 | |
| 001 | 000045882011 | |
| 005 | 20160920172734 | |
| 008 | 160919s2016 nyua b 001 0 eng d | |
| 020 | ▼a 9781493936625 | |
| 035 | ▼a (OCoLC)950464796 | |
| 040 | ▼a GW5XE ▼b eng ▼e rda ▼e pn ▼c GW5XE ▼d YDXCP ▼d SCB ▼d OCLCF ▼d SCB ▼d AZU ▼d BUF ▼d KUB ▼d 211009 | |
| 049 | ▼a KUBA | |
| 050 | 4 | ▼a QH603.C4 |
| 082 | 0 4 | ▼a 571.6 ▼2 23 |
| 084 | ▼a 571.6 ▼2 DDCK | |
| 090 | ▼a 571.6 ▼b G2112 | |
| 245 | 0 0 | ▼a Gap junction protocols / ▼c edited by Mathieu Vinken, Scott R. Johnstone. |
| 260 | ▼a New York, NY : ▼b Humana Press : ▼b Springer, ▼c c2016. | |
| 300 | ▼a xiii, 235 p. : ▼b ill. (some col.) ; ▼c 27 cm. | |
| 490 | 1 | ▼a Springer protocols |
| 490 | 1 | ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 1437 |
| 504 | ▼a Includes bibliographical references and index. | |
| 505 | 0 | ▼a Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction -- DNA Methylation Analysis of Human Tissue-Specific Connexin Genes -- Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis -- Immunohisto- and Cytochemistry Analysis of Connexins -- Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells -- Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor -- Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells -- Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis -- Scrape Loading/Dye Transfer Assay -- Microinjection Technique for Assessment of Gap Junction Function -- Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling -- Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro -- Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging -- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells -- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels -- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance. |
| 520 | ▼a Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings. | |
| 650 | 0 | ▼a Gap junctions (Cell biology) ▼v Laboratory manuals. |
| 650 | 2 | ▼a Gap Junctions. |
| 700 | 1 | ▼a Vinken, Mathieu. |
| 700 | 1 | ▼a Johnstone, Scott R. |
| 830 | 0 | ▼a Springer protocols. |
| 830 | 0 | ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 1437. |
| 945 | ▼a KLPA |
소장정보
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 과학도서관/Sci-Info(2층서고)/ | 청구기호 571.6 G2112 | 등록번호 121237606 | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
컨텐츠정보
책소개
1. Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction
Micha?l Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken
2. DNA Methylation Analysis of Human Tissue-Specific Connexin Genes
Xiaoming Liu and Canxia Xu
3. Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis
Joost Willebrords, Micha?l Maes, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken
4. Immunohisto- and Cytochemistry Analysis of Connexins
Bruno Cogliati, Micha?l Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara Crespo Yanguas, and Mathieu Vinken
5. Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells
Miranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, and Brant E. Isakson
6. Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor
Mark Kibschull, Stephen J. Lye, and Oksana Shynlova
7. Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells
Alexander W. Lohman, Adam C. Straub, and Scott R. Johnstone
8. Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis
Stephanie Fowler, Mark Akins, and Steffany A.L. Bennett
9. Scrape Loading/Dye Transfer Assay
Pavel Babica, Iva Sovadinov?, and Brad L. Upham
10. Microinjection Technique for Assessment of Gap Junction Function
Michael D. Fridman, Jun Liu, Yu Sun, and Robert M. Hamilton
11. Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling
Elke Decrock, Marijke De Bock, Diego De Baere, Delphine Hoorelbeke, Nan Wang, and Luc Leybaert
12. Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro
Maria Kuzma-Kuzniarska, Clarence Yapp, and Philippa A. Hulley
13. Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging
Song Yang and Wen-Hong Li
14. A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells
Joseph P. Stains and Roberto Civitelli
15. Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels
정보제공 :
목차
1. Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction Michael Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken 2. DNA Methylation Analysis of Human Tissue-Specific Connexin Genes Xiaoming Liu and Canxia Xu 3. Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis Joost Willebrords, Michael Maes, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken 4. Immunohisto- and Cytochemistry Analysis of Connexins Bruno Cogliati, Michael Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara Crespo Yanguas, and Mathieu Vinken 5. Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells Miranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, and Brant E. Isakson 6. Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor Mark Kibschull, Stephen J. Lye, and Oksana Shynlova 7. Identification of Connexin43 Phosphorylation and S -Nitrosylation in Cultured Primary Vascular Cells Alexander W. Lohman, Adam C. Straub, and Scott R. Johnstone 8. Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis Stephanie Fowler, Mark Akins, and Steffany A.L. Bennett 9. Scrape Loading/Dye Transfer Assay Pavel Babica, Iva Sovadinova, and Brad L. Upham 10. Microinjection Technique for Assessment of Gap Junction Function Michael D. Fridman, Jun Liu, Yu Sun, and Robert M. Hamilton 11. Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling Elke Decrock, Marijke De Bock, Diego De Baere, Delphine Hoorelbeke, Nan Wang, and Luc Leybaert 12. Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro Maria Kuzma-Kuzniarska, Clarence Yapp, and Philippa A. Hulley 13. Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging Song Yang and Wen-Hong Li 14. A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells Joseph P. Stains and Roberto Civitelli 15. Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels Jegan Iyyathurai, Bernard Himpens, Geert Bultynck, and Catheleyne D'hondt 16. Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance Richard D. Veenstra
정보제공 :