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Exosomes and microvesicles : methods and protocols

Exosomes and microvesicles : methods and protocols (8회 대출)

자료유형
단행본
개인저자
Hill, Andrew F. (Andrew Francis).
서명 / 저자사항
Exosomes and microvesicles : methods and protocols / edited by Andrew F. Hill.
발행사항
New York :   Humana Press :   Springer,   c2017.  
형태사항
xii, 228 p. : ill (some col.) ; 27 cm.
총서사항
Methods in Molecular Biology,1064-3745 ; 1545
ISBN
9781493967261 1493967266
내용주기
Methods to analyze EVs / Bernd Giebel and Clemens Helmbrecht -- Tunable resistive pulse sensing for the characterization of extracellular vesicles / Sybren L.N. Maas, Marike L.D. Broekman, and Jeroen de Vrij -- Immuno-characterization of exosomes using nanoparticle tracking analysis / Kym McNicholas and Michael Z. Michael -- Imaging and quantification of extracellular vesicles by transmission electron microscopy / Romain Linares, Sisareuth Tan, Céline Gounou, and Alain R. Brisson -- Quantitative analysis of exosomal miRNA via qPCR and digital PCR / Shayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill -- Small RNA library construction for exosomal RNA from biological samples for the Ion Torrent PGM and Ion S5TM system / Lesley Cheng and Andrew F. Hill -- Protocol for isolation and proteomic characterization of distinct extracellular vesicle subtypes by sequential centrifugal ultrafiltration / Rong Xu, Richard J. Simpson, and David W. Greening -- Multiplexed phenotyping of small extracellular vesicles using protein microarray (EV Array) / Rikke Bæk and Malene Møller Jørgensen -- Purification and analysis of exosomes released by mature cortical neurons following synaptic activation / Karine Laulagnier, Charlotte Javalet, Fiona J. Hemming, and Rémy Sadoul -- Method for isolation of extracellular vesicles and characterization of exosomes from brain extracellular space / Rocío Perez-Gonzalez, Sebastien A. Gauthier, Asok Kumar, Mitsuo Saito, Mariko Saito, and Efrat Levy -- Isolation of exosomes and microvesicles from cell culture systems to study prion transmission / Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann, Laurent Schaeffer, Graça Raposo, and Didier Vilette -- Isolation of platelet-derived extracellular vesicles / Maria Aatonen, Sami Valkonen, Anita Böing, Yuana Yuana, Rienk Nieuwland, and Pia Siljander -- Bioinformatics tools for extracellular vesicles research / Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, and Suresh Mathivanan -- Preparation and isolation of siRNA-loaded extracellular vesicles / Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir E.L. Andaloussi, and Matthew J.A. Wood -- Interaction of extracellular vesicles with endothelial cells under physiological flow conditions / Susan M. van Dommelen, Margaret Fish, Arjan D. Barendrecht, Raymond M. Schiffelers, Omolola Eniola-Adefeso, and Pieter Vader -- Flow cytometric analysis of extracellular vesicles / Aizea Morales-Kastresana and Jennifer C. Jones.
서지주기
Includes bibliographical references and index.
일반주제명
Extracellular space --Laboratory manuals.
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090 ▼a 571.6 ▼b E963
245 0 0 ▼a Exosomes and microvesicles : ▼b methods and protocols / ▼c edited by Andrew F. Hill.
260 ▼a New York : ▼b Humana Press : ▼b Springer, ▼c c2017.
300 ▼a xii, 228 p. : ▼b ill (some col.) ; ▼c 27 cm.
490 1 ▼a Methods in Molecular Biology, ▼x 1064-3745 ; ▼v 1545
490 1 ▼a Springer protocols
504 ▼a Includes bibliographical references and index.
505 0 0 ▼t Methods to analyze EVs / ▼r Bernd Giebel and Clemens Helmbrecht -- ▼t Tunable resistive pulse sensing for the characterization of extracellular vesicles / ▼r Sybren L.N. Maas, Marike L.D. Broekman, and Jeroen de Vrij -- ▼t Immuno-characterization of exosomes using nanoparticle tracking analysis / ▼r Kym McNicholas and Michael Z. Michael -- ▼t Imaging and quantification of extracellular vesicles by transmission electron microscopy / ▼r Romain Linares, Sisareuth Tan, Céline Gounou, and Alain R. Brisson -- ▼t Quantitative analysis of exosomal miRNA via qPCR and digital PCR / ▼r Shayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill -- ▼t Small RNA library construction for exosomal RNA from biological samples for the Ion Torrent PGM and Ion S5TM system / ▼r Lesley Cheng and Andrew F. Hill -- ▼t Protocol for isolation and proteomic characterization of distinct extracellular vesicle subtypes by sequential centrifugal ultrafiltration / ▼r Rong Xu, Richard J. Simpson, and David W. Greening -- ▼t Multiplexed phenotyping of small extracellular vesicles using protein microarray (EV Array) / ▼r Rikke Bæk and Malene Møller Jørgensen -- ▼t Purification and analysis of exosomes released by mature cortical neurons following synaptic activation / ▼r Karine Laulagnier, Charlotte Javalet, Fiona J. Hemming, and Rémy Sadoul -- ▼t Method for isolation of extracellular vesicles and characterization of exosomes from brain extracellular space / ▼r Rocío Perez-Gonzalez, Sebastien A. Gauthier, Asok Kumar, Mitsuo Saito, Mariko Saito, and Efrat Levy -- ▼t Isolation of exosomes and microvesicles from cell culture systems to study prion transmission / ▼r Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann, Laurent Schaeffer, Graça Raposo, and Didier Vilette -- ▼t Isolation of platelet-derived extracellular vesicles / ▼r Maria Aatonen, Sami Valkonen, Anita Böing, Yuana Yuana, Rienk Nieuwland, and Pia Siljander -- ▼t Bioinformatics tools for extracellular vesicles research / ▼r Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, and Suresh Mathivanan -- ▼t Preparation and isolation of siRNA-loaded extracellular vesicles / ▼r Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir E.L. Andaloussi, and Matthew J.A. Wood -- ▼t Interaction of extracellular vesicles with endothelial cells under physiological flow conditions / ▼r Susan M. van Dommelen, Margaret Fish, Arjan D. Barendrecht, Raymond M. Schiffelers, Omolola Eniola-Adefeso, and Pieter Vader -- ▼t Flow cytometric analysis of extracellular vesicles / ▼r Aizea Morales-Kastresana and Jennifer C. Jones.
650 0 ▼a Extracellular space ▼v Laboratory manuals.
700 1 ▼a Hill, Andrew F. ▼q (Andrew Francis).
830 0 ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 1545.
830 0 ▼a Springer protocols (Series).
945 ▼a KLPA

소장정보

No. 소장처 청구기호 등록번호 도서상태 반납예정일 예약 서비스
No. 1 소장처 과학도서관/Sci-Info(2층서고)/ 청구기호 571.6 E963 등록번호 121239315 (8회 대출) 도서상태 대출가능 반납예정일 예약 서비스 B M

컨텐츠정보

책소개

Preface...
Table of Contents...
Contributing Authors...

1. Methods to Analyze EVs
Bernd Giebel and Clemens Helmbrecht

2. Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles
Sybren L. N. Maas, Marike L.D. Broekman, and Jeroen de Vrij

3. Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis
Kym McNicholas and Michael Z. Michael

4. Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy
Romain Linares, Sisareuth Tan, C?line Gounou, and Alain R. Brisson

5. Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR
Shayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill

6. Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM(TM) System
Lesley Cheng and Andrew F. Hill

7. A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration
Rong Xu, Richard J. Simpson, and David W. Greening

8. Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array)
Rikke B k and Malene M ller J rgensen

9. Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation
Karine Laulagnier, Charlotte Javalet, Fiona Hemming, and R?my Sadoul

10. A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space
R. Perez-Gonzalez, S.A. Gauthier, A. Kumar, M. Saito, M. Saito, and E. Levy

11. Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission
Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann, Laurent Schaeffer, Gra?a Raposo and Didier Vilette

12. Isolation of Platelet-Derived Extracellular Vesicles
Maria Aatonen, Sami Valkonen, Anita B?ing, Yuana Yuana, Rienk Nieuwland and Pia Siljander

13. Bioinformatics Tools for Extracellular Vesicles Research
Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, and Suresh Mathivanan

14. Preparation and Isolation of siRNA-Loaded Extracellular Vesicles
Pieter Vader, Imre M?ger, Yi Lee, Joel Z. Nordin, Samir EL Andaloussi, and Matthew J.A. Wood

15. Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions
S.M. van Dommelen, M. Fish, A.D. Barendrecht, R.M. Schiffelers, O. Eniola-Adefeso, P. Vader


정보제공 : Aladin

목차

Methods to Analyze EVs
Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles
Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis
Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy
Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR
Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ System
A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration
Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array)
Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation
A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space
Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission
Isolation of Platelet-Derived Extracellular Vesicles
Bioinformatics Tools for Extracellular Vesicles Research
Preparation and Isolation of siRNA-Loaded Extracellular Vesicles
Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions.

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