| 000 | 00000cam u2200205 a 4500 | |
| 001 | 000045906028 | |
| 005 | 20170522133631 | |
| 008 | 170519s2017 nyua b 001 0 eng d | |
| 020 | ▼a 9781493966325 (hbk.) | |
| 020 | ▼a 9781493966349 (ebk.) | |
| 035 | ▼a (OCoLC)986720310 | |
| 040 | ▼a ERH ▼b cat ▼e rda ▼c ERH ▼d ERH ▼d KUB ▼d 211009 | |
| 049 | ▼a KUBA | |
| 082 | 0 4 | ▼a 615.7922 ▼2 23 |
| 084 | ▼a 615.7922 ▼2 DDCK | |
| 090 | ▼a 615.7922 ▼b A629 | |
| 245 | 0 0 | ▼a Antibiotics : ▼b methods and protocols / ▼c edited by Peter Sass, Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive Compounds, University of Tübingen, Tübingen, Germany. |
| 260 | ▼a New York : ▼b Humana Press : ▼b Springer, ▼c c2017. | |
| 300 | ▼a xi, 358 p. : ▼b ill. (some col.) ; ▼c 27 cm. | |
| 490 | 1 | ▼a Methods in molecular biology ; ▼v 1520 |
| 490 | 1 | ▼a Springer protocols |
| 504 | ▼a Includes bibliographical references and index. | |
| 505 | 8 0 | ▼a Conté: Antibiotics: precious goods in changing times -- Mining bacterial genomes for secondary metabolite gene clusters -- Production of antimicrobial compounds by fermentation -- Structure elucidation of antibiotics by NMR spectroscopy -- Computer-aided drug design methods -- Cytotoxicity assays as predictors of the safety and efficacy of antimicrobial agents -- Application of a Bacillus subtilis whole-cell biosensor (PliaI-lux) for the identification of cell wall active antibacterial compounds -- Determination of bacterial membrane impairment by antimicrobial agents -- Mass-sensitive biosensor systems to determine the membrane interaction of analytes -- Measurement of cell membrane fluidity by Laurdan GP: fluorescence spectroscopy and microscopy -- In vitro assays to identify antibiotics targeting DNA metabolism -- Fluorescence-based real-time activity assays to identify RNase P inhibitors -- Reporter gene-based screening for TPP riboswitch activators -- Cell-based fluorescent screen to identify inhibitors of bacterial translation initiation -- Bacterial histidine kinases : overexpression, purification, and inhibitor screen -- Expression profiling of antibiotic-resistant bacteria obtained by laboratory evolution -- Sample preparation for mass-spectrometry based absolute protein quantification in antibiotic stress research -- Label-free quantitation of ribosomal proteins from Bacillus subtilis for antibiotic research -- Functional metagenomics to study antibiotic resistance -- Epidemiological surveillance and typing methods to track antibiotic resistant strains using high throughput sequencing |
| 520 | ▼a "This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls." -- Springer. | |
| 700 | 1 | ▼a Sass, Peter. |
| 830 | 0 | ▼a Methods in molecular biology (Humana Press) ; ▼v 1520. |
| 830 | 0 | ▼a Springer Protocols. |
| 945 | ▼a KLPA |
소장정보
| No. | 소장처 | 청구기호 | 등록번호 | 도서상태 | 반납예정일 | 예약 | 서비스 |
|---|---|---|---|---|---|---|---|
| No. 1 | 소장처 과학도서관/Sci-Info(2층서고)/ | 청구기호 615.7922 A629 | 등록번호 121240296 | 도서상태 대출가능 | 반납예정일 | 예약 | 서비스 |
컨텐츠정보
책소개
Part I: Production and Design
1. Antibiotics - Precious Goods in Changing Times
Peter Sass
2. Mining Bacterial Genomes for Secondary Metabolite Gene Clusters
Martina Adamek, Marius Spohn, Evi Stegmann, and Nadine Ziemert
3. Production of Antimicrobial Compounds by Fermentation
Henrik Harms, Gabriele M. K?nig and Till F. Sch?berle
4. Structure Elucidation of Antibiotics by NMR Spectroscopy
Georgios Daletos, Elena Ancheeva, Raha Orfali, Victor Wray, and Peter Proksch
5. Computer-Aided Drug Design Methods
Wenbo Yu and Alexander D. MacKerell, Jr.
6. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents
Alexander Zipperer and Dorothee Kretschmer
Part II: Mode of Action
7. Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds
Carolin Martina Kobras, Thorsten Mascher, and Susanne Gebhard
8. Determination of Bacterial Membrane Impairment by Antimicrobial Agents
Miriam Wilmes and Hans-Georg Sahl
9. Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes
Sebastian G. Ho and Gerd Bendas
10. Measurement of Cell Membrane Fluidity by Laurdan GP: Fluorescence Spectroscopy and Microscopy
Kathi Scheinpflug, Oxana Krylova and Henrik Strahl
11. In vitro Assays to Identify Antibiotics Targeting DNA Metabolism
12. Fluorescence-based real-time Activity Assays to Identify RNase P inhibitorsYu Chen, Xin Liu, Nancy Wu, and Carol A. Fierke
13. Reporter Gene-based Screening for TPP Riboswitch Activators
Christina E. L?nse and G?nter Mayer
14. Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation InitiationFederica Briani
15. Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen
Mike Gajdiss, Michael T?rck, and Gabriele Bierbaum
Part III: Response and Susceptibility
16. Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution
Shingo Suzuki, Takaaki Horinouchi, and Chikara Furusawa 17. Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress ResearchFlorian Bonn, Sandra Maass, and D?rte Becher
18. Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research
Sina Sch?kermann, Pascal Prochnow, and Julia E. Bandow
19. Functional Metagenomics to Study Antibiotic Resistance
Manish Boolchandani, Sanket Patel, and Gautam Dantas
20. Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing
Miguel Paulo Machado, Bruno Ribeiro-Gon?alves, Mickael Silva, M?rio Ramirez, Jo?o Andr? Carri?o
정보제공 :
목차
Antibiotics - Precious Goods in Changing Times Mining Bacterial Genomes for Secondary Metabolite Gene Clusters Production of Antimicrobial Compounds by Fermentation Structure Elucidation of Antibiotics by NMR Spectroscopy Computer-Aided Drug Design Methods Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds Determination of Bacterial Membrane Impairment by Antimicrobial Agents Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and Microscopy In vitro Assays to Identify Antibiotics Targeting DNA Metabolism Fluorescence-based real-time Activity Assays to Identify RNase P inhibitors Reporter Gene-based Screening for TPP Riboswitch Activators Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation.-Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research Functional Metagenomics to Study Antibiotic Resistance Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing. .