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The Ninth International Conference on Methods in Protein Sequence Analysis was held for the first time in Asia from September 20 to September 24, 1992 in Otsu (a city near Kyoto), Japan. Approximately 400 delegates attended the meeting. Forty papers were presented orally and 147 poster presentations were discussed. Academic sessions were held from early in the morning until late in the evening. We are confident that the Conference was successful in providing up-to-date information about methods in protein sequence analysis to all participants. Moreover, with the knowledge and understanding of the present standard of various methods of analysis that are being used and will be used, we were able to clarify areas that need to be evaluated, to be improved and be explored further. Major topics in the Conference mostly covered areas in the methodology of protein sequence analysis, such as: micropreparation and microsequencing of proteins, mass spectrometry, post-translational modification, prediction and database analysis, and analysis of protein structures of special interests. The evolution of genetic engineering in molecular biology has greatly accelerated the accumulation of knowledge on the amino acid sequence of novel proteins regardless of whether they are expressed or not expressed in living organisms. In the early stage of accumulation of structural information, the amino acid sequence itself is worthy of notice.

The Ninth International Conference on Methods in Protein Sequence Analysis was held for the first time in Asia from September 20 to September 24, 1992 in Otsu (a city near Kyoto), Japan. Approximately 400 delegates attended the meeting. Forty papers were presented orally and 147 poster presentations were discussed. Academic sessions were held from early in the morning until late in the evening. We are confident that the Conference was successful in providing up-to-date information about methods in protein sequence analysis to all participants. Moreover, with the knowledge and understanding of the present standard of various methods of analysis that are being used and will be used, we were able to clarify areas that need to be evaluated, to be improved and be explored further. Major topics in the Conference mostly covered areas in the methodology of protein sequence analysis, such as: micropreparation and microsequencing of proteins, mass spectrometry, post-translational modification, prediction and database analysis, and analysis of protein structures of special interests. The evolution of genetic engineering in molecular biology has greatly accelerated the accumulation of knowledge on the amino acid sequence of novel proteins regardless of whether they are expressed or not expressed in living organisms. In the early stage of accumulation of structural information, the amino acid sequence itself is worthy of notice.

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CONTENTS
MICROPREPARATION AND MICROSEQUENCING
  Capillary liquid chromatography : a tool for protein structural analysis / R. L. Moritz ; R. J. Simpson = 3
  Routine animo acid sequencing on 2D-gel separated proteins : a protein elution and concentration gel system / J. Vandekerckhove ; M. Rider ; H.-H. Rasmussen ; S. De Boeck ; M. Puype ; J. Van Damme ; B. Gesser ; J. Celis = 13
  Capillary electrophoresis in structural characterization of polypeptides = T. Bergman = 21
  Sensitization of gas-phase protein sequencer using fluorescein isothiocyanate(FITC) / K. Muramoto ; K. Nokihara ; A. Ueda ; H. Kamiya = 29
  Thiobenzoylation method of protein sequencing : gas chromatography／mass spectrometric detection of 5-acetoxy-2-phenylthiazoles / M. L. Stolowitz ; C.-S. Kim ; S. R. Marsh ; L. Hood = 37
  Deblocking and subsequent microsequence analysis of N-terminally blocked proteins immobilized on PVDF membrane / S. Tsunasawa ; H. Hirano = 45
  Development of novel C-terminal sequencing methods / A. Tsugita ; K. Takamoto ; H. Iwadate ; M. Kamo ; H. Yano ; N. Miyatake ; K. Satake = 55
  Automated C-terminal sequencing of peptides and proteins / J. M. Bailey ; M. Rusnak ; J. E. Shively = 63
  A new chemical approach to C-terminal microsequence analysis via the thiohydantoin / A. S. Inglis ; C. De Luca = 71
  Cyanogen bromide cleavage of proteins on blots and subsequent separation of the Fragments by polyacrylamide gel electrophoresis directly from those blots / G. Frank ; U. Redweik ; M. Rumbeli ; G. Schoedon ; N. Blau = 79
  Identification of the disulfide bonds of the human complement component C3 / J. Schaller ; S. Sohndel ; E. E. Rickli = 87
  A novle protease from jack-bean seeds : asparaginyl endopeptidase / S. Ishii ; Y. Abe ; M. Mitta ; H. Matsushita ; I. Kato = 95
  Lysine-specific serine protease from a Achromobacter lyticus : its substrate specificity and comparison with trypsin = 101
  Structure and function of snake venom metalloproteinase family / S. Iwanaga ; H. Takeya = 107
MASS SPECTROMETRY
  Recent advances in protein sequencing by mass spectrometry. Introduction and overview / K. Biemann = 119
  Sequence analysis of peptides presented to the immune system by class Ⅰ and class Ⅱ MHC molecules / D. F. Hunt ; J. Shabanowitz ; H. Michel ; A. L. Cox ; T. Dickinson ; T. Davis ; W. Bodnar ; R. A. Henderson ; N. Sevilir ; V. H. Engelhard ; K. Sakaguchi ; E. Appella ; H. M. Grey ; A. Sette = 127
  High precision mass spectrometry wtih the ion trap mass spectrometer / R. G. Cooks ; K. A. Cox ; J. D. Williams = 135
  Electrospray-mass spectrometry, an emerging methodology for elucidating structure-function relationships of proteins / K. A. Walsh ; L. H. Ericsson ; K. Resing ; R. S. Johnson = 143
  A general strategy for the use of mass spectrometric molecular weight information in protein purification and sequence determination / P. Roepstorff ; P. Hψjrup = 149
  Determination of posttranslational modiffications by mass spectrometry / T. Takao and Y. Shimonishi = 157
POST-TRANSLATIONAL MODIFICATIONS
  Post-translational modifications of proteins / R. G. Krishna ; F. Wold = 167
  N-Terminal acetylation of mutationally altered from of iso-l-cytochromes c in normal and natl-strans deficient in the major N-termianl acetyl transferase of the yeast Saccharomyces cerevisiae / F. Sherman ; R. P. Moerschell ; S. Tsunasawa ; R. Sternglanz = 173
  Posttranslational glutamylation of several brain tubulin isotypes : structure of the polyglutamyl side chain / V. Redeker ; J. -P. Le Caer ; J. Rossier = 183
  Protein anchoring to membrane by glycosylphosphatidylinositol : Determination of the COOH-terminal signal sequence and GPI-attachment site in bovine liver 5'-nucleotidase / H. Ikezawa = 191
  Pyridylamination for sensitive analysis of sugar chains of glycoproteins / S. Hase = 195
  Sequence analysis of phosphopeptides and its application for the determination of phosphorylated sites of proteins / T. Uchida ; A. Omori ; K. Ishiguro ; K. Sato = 199
PREDICTION AND DATABASE ANALYSIS
  Prediction of protein structure from multiple sequence alignment / G. J. Barton ; R. B. Russell and C. D. Livingstone = 209
  Sequence patterns that characterize protein families with a common fold / M. S. Johnson ; T. L. Blundell = 221
  Domains and modules of proteins / M. G o ^ ; M. Nosaka ; S. Tomoda = 229
  Homology search and prediction of biological function of protein from amino acid sequences / H. Toh = 233
  Amino acid sequence comparison as an aid to determining evolutionary arigins / R. F. Doolittle / 241
  Protein databases constructed by quantitative two-dimensional gel electrophoresis J. I. Garrels ; B. R. Franza, Jr. ; S. D. Patterson ; K. Latham ; D. Solter ; C. Chang ; G. Latter = 247
PROTEIN STRUCTURES OF SPECIAL INTERESTS
  Biochemistry of a natriuretic peptide family / H. Matsuo = 257
  Active, activin-binding protein(follistation)and activin receptor / H. Sugino ; T. Nakamura ; K. Sugino ; K. Titani = 261
  Do well-populated intermediates represent the pathway of protein folding? / J.-Y. Chang = 269
  Alcohol dehydrogenases : patterns of protein evolution / H. J o ·· rnvall ; O. Danielsson ; J. -O. H o ·· o ·· g ; B. Persson = 275
  Structure and post-translational modification of the lipolyl domain of 2-oxo acid dehydrogenase complexes : a new family of protein domains / R. N. Perham ; N. G. Wallis ; S. M. Brocklehurst ; F. Dardel ; A. L. Davis ; E. D. Laue = 283
  Structure analysis of the topography and molecular organization of protein-RNA complexes as revealed in ribosomes / B. Wittmann-Liebold = 289
Index = 307