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| 005 | 20151119173841 | |
| 008 | 151119s2014 nyua b 001 0 eng d | |
| 010 | ▼a 2014942725 | |
| 015 | ▼a GBB474840 ▼2 bnb | |
| 020 | ▼a 9781493911387 (alk. paper) | |
| 020 | ▼a 1493911384 (alk. paper) | |
| 020 | ▼z 9781493911394 (eBook) | |
| 020 | ▼z 1493911392 (eBook) | |
| 035 | ▼a (KERIS)REF000017806609 | |
| 040 | ▼a NLM ▼b eng ▼c NLM ▼e rda ▼d YDXCP ▼d BTCTA ▼d UKMGB ▼d HNK ▼d FTU ▼d NUI ▼d OCLCF ▼d OCLCO ▼d DLC ▼d 211009 | |
| 050 | 0 0 | ▼a QP552.N67 ▼b N685 2014 |
| 082 | 0 0 | ▼a 616.8/31071 ▼2 23 |
| 084 | ▼a 616.831071 ▼2 DDCK | |
| 090 | ▼a 616.831071 ▼b N899 | |
| 130 | 0 | ▼a Notch signaling (Bellen) |
| 245 | 1 0 | ▼a Notch signaling : ▼b methods and protocols / ▼c edited by Hugo J. Bellen, Department of Molecular and Human Genetics, Program in Developmental Biology, Department of Neuroscience, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA, Shinya Yamamoto, Department of Molecular and Human Genetics, Program in Developmental Biology, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA. |
| 260 | ▼a New York : ▼b Humana Press : ▼b Springer, ▼c 2014. | |
| 300 | ▼a xi, 351 p. : ▼b ill. (some col.) ; ▼c 26 cm. | |
| 490 | 1 | ▼a Methods in molecular biology, ▼x 1064-3745 ; ▼v 1187 |
| 490 | 1 | ▼a Springer protocols, ▼x 1949-2448 |
| 504 | ▼a Includes bibliographical references and index. | |
| 505 | 0 | ▼t Introduction to notch signaling / ▼r Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen -- ▼t Genetic screens to identify new notch pathway mutants in Drosophila / ▼r Nikolaos Giagtzoglou -- ▼t Structure-function analysis of Drosophila notch using genomic tescue transgenes / ▼r Jessica Leonardi and Hamed Jafar-Nejad -- ▼t Overview of genetic tools and techniques to study notch signaling in mice / ▼r Thomas Gridley and Andrew K. Groves -- ▼t Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster / ▼r Emiliana Tognon and Thomas Vaccari -- ▼t Antibody uptake assay and in vivo imaging to study intracellular trafficking of notch and delta in Drosophila / ▼r Lydie Couturier and François Schweisguth -- ▼t Tracking trafficking of notch and its ligands in mammalian cells / ▼r Patricia Chastagner and Christel Brou -- ▼t Visualizing notch signaling in vivo in Drosophila tissues / ▼r Benjamin E. Housden, Jinghua Li, and Sarah J. Bray -- ▼t Monitoring notch activity in the mouse / ▼r Swananda Marathe and Lavinia Alberi -- ▼t Notch signaling assays in Drosophila cultured cell lines / ▼r Jinghua Li, Benjamin E. Housden, and Sarah J. Bray -- ▼t Monitoring notch activation in cultured mammalian cells : transcriptional reporter assays / ▼r Ma. Xenia G. Ilagan and Raphael Kopan -- ▼t Monitoring notch activation in cultured mammalian cells : luciferase complementation imaging assays / ▼r Ma. Xenia G. Ilagan and Raphael Kopan -- ▼t Visualization of notch signaling oscillation in cells and tissues / ▼r Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama -- ▼t Proteomic analysis of the notch interactome / ▼r K. G. Guruharsha ... [et al.] -- ▼t Bacterial expression and in vitro refolding of limited fragments of the notch receptor and its ligands / ▼r Pat Whiteman, Christina Redfield, and Penny A. Handford -- ▼t Analyzing the posttranslational modification status of notch using mass spectrometry / ▼r Shinako Kakuda and Robert S. Haltiwanger -- ▼t Assay to probe proteolytic processing of notch by [gamma]-secretase / ▼r Lutgarde Serneels, Ina Tesseur, and Bart De Strooper -- ▼t Analyzing the nuclear complexes of notch signaling by electrophoretic mobility shift assay / ▼r Kelly L. Arnett and Stephen C. Blacklow -- ▼t Identifying direct notch transcriptional targets using the GSI-washout assay / ▼r Will Bailis, Yumi Yashiro-Ohtani, and Warren S. Pear -- ▼t Probing the epigenetic status at notch target genes / ▼r Robert Liefke and Tilman Borggrefe -- ▼t Notch-ligand binding assays in Drosophila cells / ▼r Aiguo Xu and Kenneth D. Irvine -- ▼t Modeling notch signaling : a practical tutorial / ▼r Pau Formosa-Jordan and David Sprinzak -- ▼t Small molecules that inhibit notch signaling / ▼r Gerdien E. De Kloe and Bart De Strooper -- ▼t Application and evaluation of anti-notch antibodies to modulate notch signaling / ▼r Wendy R. Gordon and Jon C. Aster -- ▼t Application of anti-ligand antibodies to inhibit notch signaling / ▼r Jun-ichiro Koga and Masanori Aikawa. |
| 650 | 0 | ▼a Notch genes ▼v Laboratory manuals. |
| 650 | 0 | ▼a Notch proteins ▼v Laboratory manuals. |
| 650 | 1 2 | ▼a Receptors, Notch ▼v Laboratory Manuals. |
| 650 | 1 2 | ▼a Signal Transduction ▼v Laboratory Manuals. |
| 700 | 1 | ▼a Bellen, Hugo J. |
| 700 | 1 | ▼a Yamamoto, Shinya. |
| 830 | 0 | ▼a Methods in molecular biology (Clifton, N.J.) ; ▼v v. 1187. |
| 830 | 0 | ▼a Springer protocols (Series). |
| 945 | ▼a KLPA |
Holdings Information
| No. | Location | Call Number | Accession No. | Availability | Due Date | Make a Reservation | Service |
|---|---|---|---|---|---|---|---|
| No. 1 | Location Science & Engineering Library/Sci-Info(Stacks2)/ | Call Number 616.831071 N899 | Accession No. 121234805 (4회 대출) | Availability Available | Due Date | Make a Reservation | Service |
Contents information
Book Introduction
Notch Signaling: Methods and Protocols focuses on molecular, biochemical and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor.
New feature
Notch Signaling: Methods and Protocols focuses on molecular, biochemical, and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage, and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor.
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Table of Contents
1. Introduction to Notch Signaling Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen 2. Genetic Screens to Identify New Notch Pathway Mutants in Drosophila Nikolaos Giagtzoglou 3. Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes Jessica Leonardi and Hamed Jafar-Nejad 4. Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice Thomas Gridley and Andrew K. Groves 5. Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster Emiliana Tognon and Thomas Vaccari 6. Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila Lydie Couturier and Francois Schweisguth 7. Tracking Trafficking of Notch and its Ligands in Mammalian Cells Patricia Chastagner and Christel Brou 8. Visualizing Notch Signaling in vivo in Drosophila Tissues Benjamin E. Housden, Jinghua Li, and Sarah J. Bray 9. Monitoring Notch Activity in the Mouse Lavinia Alberi and Swananda Marathe 10. Notch Signaling Assays in Drosophila Cultured Cell Lines Jinghua Li, Benjamin E. Housden, and Sarah J. Bray 11. Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays Ma. Xenia G. Ilagan and Raphael Kopan 12. Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays Ma. Xenia G. Ilagan and Raphael Kopan 13. Visualization of Notch Signaling Oscillation in Cells and Tissues Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama 14. Proteomic Analysis of the Notch Interactome K. G. Guruharsha, Kazuya Hori, Robert A. Obar, and Spyros Artavanis-Tsakonas 15. Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands Pat Whiteman, Christina Redfield and Penny A. Handford 16. Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry Shinako Kakuda and Robert S. Haltiwanger 17. Assay to Probe Proteolytic Processing of Notch by Gamma-secretase Lutgarde Serneels, Ina Tesseur and Bart De Strooper 18. Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay Kelly L. Arnett and Stephen C. Blacklow 19. Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay Will Bailis, Yumi Yashiro-Ohtani and Warren S. Pear 20. Probing the Epigenetic Status at Notch Target Genes Tilman Borggrefe and Robert Liefke 21. Notch-ligand Binding Assays in Drosophila Cells Aiguo Xu and Kenneth D. Irvine 22. Modeling Notch Signaling: A Practical Tutorial Pau Formosa-Jordan and David Sprinzak 23. Small Molecules that Inhibit Notch Signaling Gerdien E. De Kloe and Bart De Strooper 24. Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling Wendy R. Gordon and Jon C. Aster 25. Application of Anti-ligand Antibodies to Inhibit Notch Signaling Jun-ichiro Koga and Masanori Aikawa
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